MicroRNA-361-5p Inhibits Tumorigenesis and the EMT of HCC by Targeting Twist1
| Autor: | Jun Weng, Liang-Chun Yin, Lixin Liu, Can-Liang Tan, Xiaoping Huang, Ninglei Li, Gang Xiao, Feng-Jiao Xie, Rui Zhou |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Carcinoma Hepatocellular Epithelial-Mesenchymal Transition Article Subject Carcinogenesis Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences 0302 clinical medicine law Cell Line Tumor microRNA medicine Humans General Immunology and Microbiology Transition (genetics) Cell growth Liver Neoplasms Twist-Related Protein 1 Nuclear Proteins General Medicine medicine.disease digestive system diseases Blot MicroRNAs 030104 developmental biology Liver Cell culture 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Medicine Suppressor Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2020 (2020) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | MicroRNA-361-5p (miR-361-5p) is a tumor suppressor miRNA that is dysregulated in several types of human cancer. However, the functional significance of miR-361-5p in hepatocellular carcinoma (HCC) is unclear. This study explored the biological function of miR-361-5p in regulating the progression of HCC and the underlying molecular mechanism. RT-qPCR analysis showed that miR-361-5p was downregulated in HCC tissues and cell lines. Functional analysis revealed that miR-361-5p acted as a tumor suppressor, inhibiting cell proliferation, migration, and invasion in HCC cell lines. Bioinformatics analyses identified Twist1 as a direct target of miR-361-5p, which was validated by dual-luciferase reporter assays, RT-qPCR, and western blotting. Rescue experiments indicated that Twist1 may mediate the tumor-suppressive effect of miR-361-5p in HCC cells, and this was supported by the effect of miR-361-5p on inhibiting the epithelial-mesenchymal transition (EMT) by targeting Twist1. This study is the first to suggest that miR-361-5p inhibits tumorigenesis and EMT in HCC by targeting Twist1. These findings are valuable for the diagnosis and clinical management of HCC. |
| Databáze: | OpenAIRE |
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