Two-hour Sephadex column method for assay of unconjugated estriol in serum

Autor:	L F Hofman, W G Haigh, E J Barron
Rok vydání:	1980
Předmět:	Sephadex column Chromatography Chemistry Elution Biochemistry (medical) Clinical Biochemistry Estriol Blood proteins Unconjugated estriol Rapid assay Incubation reproductive and urinary physiology hormones hormone substitutes and hormone antagonists Conjugate
Zdroj:	Clinical Chemistry. 26:309-312
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/26.2.309
Popis:	The column method of Christner and Fetter (Steroids 24: 327, 1974) has been modified to give a simple, rapid assay for unconjugated estriol in serum. Estriol is isolated from serum by being retained on a Sephadex column, while estriol conjugates and serum proteins are eluted. The sample and labeled estriol compete for antibody on the column. Antibody is eluted, removing proportional amounts of sample and labeled estriol. Our modifications include using stable serum-based standards, shortening the incubation to 10 min, complete removal of conjugates, and regeneration of the columns so that they can be used repeatedly. The assay, which can be completed in 2 h, has a mid-range interassay CV of 8.2%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::071916da0a5d5381113155130c813afd https://doi.org/10.1093/clinchem/26.2.309 Zobrazit plný text záznamu