Determination of nitrofuran metabolites in honey using a new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS
| Autor: | Elena G. Shubina, V. V. Tolmacheva, Artyom I. Grudev, A. O. Melekhin, Vladimir V. Apyari, Stanislava G. Dmitrienko |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Furazolidone
medicine.drug_class Calibration curve Nitrofurans Metabolite 02 engineering and technology 01 natural sciences Analytical Chemistry Matrix (chemical analysis) chemistry.chemical_compound Tandem Mass Spectrometry Furaltadone medicine Solid phase extraction Derivatization Nitrofuran Chromatography Magnetic Phenomena 010401 analytical chemistry Solid Phase Extraction Honey 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Indicators and Reagents 0210 nano-technology medicine.drug Chromatography Liquid |
| Zdroj: | Talanta. 230 |
| ISSN: | 1873-3573 |
| Popis: | In this study, 5-nitro-2-furaldehyde (5-NFA) was proposed as a new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the parent nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe3O4) was first used for magnetic solid phase extraction (MSPE) clean-up before the determination of nitrofuran metabolite derivatives in honey via LC–MS/MS. Main parameters affecting the derivatization and MSPE efficiency were investigated in detail and the optimal conditions were found. The method was validated using honey spiked with the four metabolites at 1, 2 and 200 μg kg−1. Recoveries of >85% were achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R2) > 0.99 in the range of 1–200 μg kg−1. Precision values expressed as relative standard deviation (RSD) were |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect