The gluconeogenic glucose-6-phosphatase gene is expressed during oxygen-limited conditions in the white shrimp Penaeus (Litopenaeus) vannamei: Molecular cloning, membrane protein modeling and transcript modulation in gills and hepatopancreas
Autor: | Alma B. Peregrino-Uriarte, Silvia Gómez-Jiménez, Laura E Hernández-Aguirre, Keni Cota-Ruiz, Gloria Yepiz-Plascencia |
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Rok vydání: | 2021 |
Předmět: |
Gills
0301 basic medicine animal structures Physiology Litopenaeus Hepatopancreas 03 medical and health sciences 0302 clinical medicine Penaeidae Complementary DNA Animals Glucose homeostasis Penaeus Cloning Molecular Gene Peptide sequence biology Gluconeogenesis Cell Biology biology.organism_classification Shrimp 030104 developmental biology Biochemistry 030220 oncology & carcinogenesis Glucose-6-Phosphatase |
Zdroj: | Journal of Bioenergetics and Biomembranes. |
ISSN: | 1573-6881 0145-479X |
Popis: | The white shrimp Penaeus (Litopenaeus) vannamei is the most economically important crustacean species cultivated in the Western Hemisphere. This crustacean shifts its metabolism to survive under extreme environmental conditions such as hypoxia, although for a limited time. Glucose-6-phosphatase (G6Pase) is a key enzyme contributing to maintain blood glucose homeostasis through gluconeogenesis and glycogenolysis. To our knowledge, there are no current detailed studies about cDNA or gene sequences of G6Pase from any crustacean reported. Herein we report the shrimp P. (L.) vannamei cDNA and gene sequences. The gene contains seven exons interrupted by six introns. The deduced amino acid sequence has 35% identity to other homolog proteins, with the catalytic amino acids conserved and phylogenetically close to the corresponding invertebrate homologs. Protein molecular modeling predicted eight transmembrane helices with the catalytic site oriented towards the lumen of the endoplasmic reticulum. G6Pase expression under normoxic conditions was evaluated in hepatopancreas, gills, and muscle and the highest transcript abundance was detected in hepatopancreas. In response to different times of hypoxia, G6Pase mRNA expression did not change in hepatopancreas and became undetectable in muscle; however, in gills, its expression increased after 3 h and 24 h of oxygen limitation, indicating its essential role to maintain glycemic control in these conditions. |
Databáze: | OpenAIRE |
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