Toward gene therapy of endometriosis: transductional and transcriptional targeting of adenoviral vectors to endometriosis cells
| Autor: | Ayman Al-Hendy, H.T. Salem, Essam Al Din M. Khalifa, Nilufar Khatoon, Zeng B. Zhu, Essam R. Othman, David T. Curiel |
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| Rok vydání: | 2008 |
| Předmět: |
Transcriptional Activation
endocrine system viruses Genetic enhancement Genetic Vectors Endometriosis Transfection medicine.disease_cause Article Virus Adenoviridae Endometrium Transduction Genetic medicine Humans Luciferase Heparanase Luciferases Cells Cultured Glucuronidase integumentary system business.industry Obstetrics and Gynecology Gene targeting Genetic Therapy medicine.disease Virology Liver Gene Targeting Cancer research Capsid Proteins Female business |
| Zdroj: | American Journal of Obstetrics and Gynecology. 199:117.e1-117.e6 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/j.ajog.2008.01.059 |
| Popis: | Objective The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. Study Design Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. Results In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. Conclusion Ad-heparanase-luc showed “endometriosis on, liver off” phenotype and is a promising vector for endometriosis gene therapy. |
| Databáze: | OpenAIRE |
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