Induction of complete desiccation tolerance in carrot (Daucus carota) embryoids
| Autor: | F.A.A. Tetteroo, Folkert A. Hoekstra, Cees M. Karssen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Physiology
Daucus carota L. Desiccation Tolerance Germination Plant Science Biology 2 4-D relative humidity Desiccation tolerance abscisic acid chemistry.chemical_compound Dry weight Botany DW Laboratorium voor Plantenfysiologie MC Abscisic acid moisture content Maturation Somatic Embryogenesis 4-D Ripening biology.organism_classification pro-embryogenic masses chemistry ABA PEMs 4- dichlorophenoxy-acetic acid embryonic structures Imbibition 2 4- dichlorophenoxy-acetic acid RH dry weight EPS Desiccation Agronomy and Crop Science Laboratory of Plant Physiology Daucus carota |
| Zdroj: | Journal of Plant Physiology 145 (1995) Journal of Plant Physiology, 145, 349-356 |
| ISSN: | 0176-1617 |
| Popis: | Summary Successful induction of complete desiccation tolerance in carrot embryoids (Daucus carota L.) depends on at least four different factors. developmental stage, abscisic acid (ABA), drying rate and rehydration mode. Embryoids may become desiccation tolerant when they have reached the torpedo stage of development. In contrast, at the earlier globular and heart stages, the embryoids never germinated after any drying treatment. Addition of at least 20 μM ABA after 7 days of culture of Pro-embryogenic masses in 2,4-dichlorophenoxyacetic acid-free B5 medium was necessary to induce complete desiccation tolerance. Less ABA resulted in desiccation tolerance of the roots only, while higher ABA concentrations retarded developmental growth. Slow drying is essential for the acquisition of complete desiccation tolerance. Slowly dried embryoids (moisture content 0.05 g H20·g-1 dry weight) had germination rate of 100&, while rapidly dried ones germinated only 0 - 30&. Initially, viable dry embryoids may suffer injury when they are imbibed in water without prehydration in water vapour. One hundred percent germination was reached by prehydration of the embryoids in moist air for 4 to 8 hours at 24 °C before imbibition in B5 medium. With the optimize protocol we were able to produce desiccation tolerant embryoids of two genotypes having completely different genetic backgrounds. |
| Databáze: | OpenAIRE |
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