Single-Stranded DNA Translocation Recordings through Solid-State Nanopores on Glass Chips at 10 MHz Measurement Bandwidth
| Autor: | Marija Drndic, David J. Niedzwiecki, Kenneth L. Shepard, Siddharth Shekar, Chen-Chi Chien |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
DNA Single-Stranded General Physics and Astronomy 02 engineering and technology 010402 general chemistry 01 natural sciences Article Nanopores chemistry.chemical_compound General Materials Science chemistry.chemical_classification business.industry Amplifier Biomolecule Bandwidth (signal processing) General Engineering Oxides Signal Processing Computer-Assisted 021001 nanoscience & nanotechnology Low-noise amplifier 0104 chemical sciences Nanopore Dwell time Membrane Semiconductors chemistry Silicon nitride Metals Optoelectronics Glass 0210 nano-technology business |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.9b04626 |
| Popis: | Accurate and low-cost analysis of biomolecules is important for many applications. This work seeks to further improve the measurement bandwidths achievable with solid-state nanopores, which have emerged as an important platform for this analysis. We report single-stranded DNA translocation recordings at a bandwidth of 10 MHz copolymers of 80 (C(20)A(20)C(20)A(20)), 90 (C(30)A(30)C(30)), and 200 (C(50)A(50)C(50)A(50)) nucleotides through Si nanopores with effective diameters of 1.4–2.1 nm and effective membrane thickness 0.5 – 8.9 nm. By optimizing glass chips with thin nanopores and by integrating them with custom-designed amplifiers based on complementary metal-oxide-semiconductor (CMOS) technology, this work demonstrates detection of translocation events as brief as 100 ns with a signal-to-noise ratio exceeding seven at a measurement bandwidth of 10 MHz. We also report data robustness and variability across 13 pores of similar size and thickness yielding a current blockade between 30 and 60 % with a mean ionic current blockade (ΔI) of ~ 3 to 9 nA and a characteristic dwell time of ~ 2 to 21 ns per nucleotide. These measurements show that characteristic translocation rates are at least ten times faster than previously recorded. We detect transient intra-event fluctuations, multiple current levels within translocation events, and variability of DNA-translocation-event signatures and durations. |
| Databáze: | OpenAIRE |
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