Biotic stress caused by in vitro co-inoculation enhances the expression of acetylcholinesterase inhibitors by fungi
| Autor: | Rodinei Augusti, Geane Pereira de Oliveira, Débora Luiza Costa Barreto, Ivanildo Evódio Marriel, Jacqueline A. Takahashi, Mauro Ramalho Silva, Ubiraci Gomes de Paula Lana |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
biology Organic Chemistry fungi food and beverages Plant Science Biotic stress biology.organism_classification Biochemistry Acetylcholinesterase High-performance liquid chromatography In vitro Analytical Chemistry Serine chemistry.chemical_compound Enzyme chemistry Biosynthesis Penicillium |
| DOI: | 10.6084/m9.figshare.16645375 |
| Popis: | Fungi are an inexhaustible source of bioactive metabolites that can be driven to find medicines for chronic diseases, as Alzheimer's disease. In the present work, five species of soil-originated fungi (Aspergillus chevalieri, Clonostachys rogersoniana, Fusarium nygamai, Penicillium sp., and Talaromyces calidicanius) were submitted to mutual biotic stress aiming at activating the expression of metabolites capable of inhibiting the enzyme acetylcholinesterase. HPLC profiles showed that the in vitro biotic stress triggered the biosynthesis of metabolite-mediated defense responses. Five compounds present in the complex co-culture matrix were identified by Paper Spray Mass Spectrometry (PS-MS). The approach enhanced the biosynthesis of acetylcholinesterase inhibitors (up to 99.6% inhibition) in comparison with the individual cultures. The mutual biotic stress between T. calidicanius and A. Chevalieri led to the biosynthesis of a pool of metabolites statistically as efficient as serine (p < 0.05), the positive control used in the experiments (99.6 and 99.1% inhibition, respectively). |
| Databáze: | OpenAIRE |
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