Commercially Available Complement Component-Depleted Sera Are Unexpectedly Codepleted of Ficolin-2
| Autor: | Moon H. Nahm, Allison M. Brady, Xiaogang Cheng, K. Aaron Geno, Alex G. Dalecki |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Serum Microbiology (medical) Complement component 2 CD46 Complement C1q Clinical Biochemistry Immunology chemical and pharmacologic phenomena Saccharomyces cerevisiae Complement receptor Biology Microbiology Streptococcus pneumoniae Biochemistry Lectins Lectin pathway Factor H biology.protein Humans Immunology and Allergy Clinical Immunology CFHR5 Complement control protein |
| Zdroj: | Clinical and Vaccine Immunology. 21:1323-1329 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00370-14 |
| Popis: | The ficolins are a family of innate pattern recognition molecules that are known to bind acetylated compounds and activate complement through the association of mannose binding lectin (MBL)/ficolin-associated serine proteases (MASPs). Their importance has more recently become appreciated, as they have been shown to play a role in a variety of disease processes from infection to autoimmunity. While studying ficolin-2-mediated complement deposition onStreptococcus pneumoniae, we found that sera depleted of C1q or other complement components were also codepleted of ficolin-2 but not ficolin-1, ficolin-3, or MBL. MBL present in C1q-depleted sera was able to mediate complement deposition onSaccharomyces cerevisiae, suggesting the presence of MASPs. We found that complement was activated on pneumococci in C1q-depleted serum only after opsonization with exogenous recombinant ficolin-2 (rFicolin-2). Also, no complement deposition was observed in C1q-depleted serum when pneumococci were opsonized with rFicolin-2 mutated at its lysine-57 residue, where MASPs are known to associate. Thus, these depleted sera are a unique tool to study ficolin-2-mediated complement pathways; however, one should be aware that ficolin-2 is absent from complement component-depleted sera. |
| Databáze: | OpenAIRE |
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