Folding and Structural Characterization of Highly Disulfide-Bonded Beetle Antifreeze Protein Produced in Bacteria
| Autor: | Brian D. Sykes, Laurie A. Graham, Yih-Cherng Liou, Cyril M. Kay, Virginia K. Walker, Peter L. Davies, Margaret E Daley |
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| Rok vydání: | 2000 |
| Předmět: |
Threonine
Protein Folding Magnetic Resonance Spectroscopy medicine.disease_cause Peptide Mapping Protein Structure Secondary law.invention Antifreeze protein law Antifreeze Proteins Escherichia coli medicine Animals Trypsin Cysteine Disulfides Protein secondary structure Chromatography High Pressure Liquid Glycoproteins biology Chemistry Circular Dichroism Nuclear magnetic resonance spectroscopy biology.organism_classification Recombinant Proteins Coleoptera Folding (chemistry) Crystallography Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Antifreeze Recombinant DNA Oxidation-Reduction Bacteria Biotechnology |
| Zdroj: | Protein Expression and Purification. 19:148-157 |
| ISSN: | 1046-5928 |
| DOI: | 10.1006/prep.2000.1219 |
| Popis: | The hyperactive antifreeze protein from the beetle, Tenebrio molitor, is an 8.5-kDa, threonine-rich protein containing 16 Cys residues, all of which are involved in disulfide bonds. When produced by Escherichia coli, the protein accumulated in the supernatant in an inactive, unfolded state. Its correct folding required days or weeks of oxidation at 22 or 4 degrees C, respectively, and its purification included the removal of imperfectly folded forms by reversed-phase HPLC. NMR spectroscopy was used to assess the degree of folding of each preparation. One-dimensional (1)H and two-dimensional (1)H total correlation spectroscopy spectra were particularly helpful in establishing the characteristics of the fully folded antifreeze in comparison to less well-folded forms. The recombinant antifreeze had no free -SH groups and was rapidly and completely inactivated by 10 mM DTT. It had a thermal hysteresis activity of 2.5 degrees C at a concentration of 1 mg/ml, whereas fish antifreeze proteins typically show a thermal hysteresis of approximately 1.0 degrees C at 10-20 mg/ml. The circular dichroism spectra of the beetle antifreeze had a superficial resemblance to those of alpha-helical proteins, but deconvolution of the spectra indicated the absence of alpha-helix and the presence of beta-structure and coil. NMR analysis and secondary structure predictions agree with the CD data and are consistent with a beta-helix model proposed for the antifreeze on the basis of its 12-amino-acid repeating structure and presumptive disulfide bond arrangement. |
| Databáze: | OpenAIRE |
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