Percutaneous penetration and absorption of parathion using human and pig skin modelsin vitroand human skin grafted onto nude mouse skin modelin vivo
| Autor: | A. Capt, O. Blanck, A. Bazire, V. Vallet, D. Josse, M. Blanck, I. Boudry, G. Lallement, C. Cruz |
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| Rok vydání: | 2008 |
| Předmět: |
Insecticides
Pathology medicine.medical_specialty Swine Skin Absorption Transplantation Heterologous Mice Nude Human skin Absorption (skin) In Vitro Techniques Toxicology Acetone Mice Nude mouse In vivo Stratum corneum medicine Animals Humans Skin Membranes Ethanol Parathion integumentary system biology Chemistry Skin Transplantation Penetration (firestop) biology.organism_classification In vitro Transplantation medicine.anatomical_structure Data Interpretation Statistical Solvents Radiopharmaceuticals Biomedical engineering |
| Zdroj: | Journal of Applied Toxicology. 28:645-657 |
| ISSN: | 1099-1263 0260-437X |
| DOI: | 10.1002/jat.1317 |
| Popis: | This study determined and compared the percutaneous penetration and absorption of an organophosphorus (OP) pesticide, parathion (PA), using three experimental skin models: namely the human abdominal- and pig-ear skin in vitro models and the Human Skin grafted onto a nude mouse (HuSki) in vivo model. The percentage of topically applied dose absorbed and the doses present in the stratum corneum and skin were systematically determined at 24 h under similar experimental conditions. The three experimental skin models were first compared. Then, the advantages of the HuSki model for in vivo PA skin absorption studies were evaluated compared with the pig in vivo model previously used by others. Lastly, the relevance of each skin model to predict the permeability of human skin to PA in vivo was assessed by comparing our results with previously published in vivo human volunteer values. It was demonstrated that (a) pig-ear skin is relevant for predicting the in vitro human abdominal skin absorption taking into account a 2-3 times higher skin permeability to PA, (b) using ethanol as the vehicle, the absorption of PA was 4-5 times higher in the HuSki model than in the pig model but supports the usefulness of the HuSki model to easy mass balance studies, (c) both human in vitro and HuSki models closely predict the in vivo human volunteer absorption at 24 h when acetone is used as a vehicle but the HuSki model overcomes the known limitations of in vitro models for studying the fate of PA in the different skin layers after topical application. |
| Databáze: | OpenAIRE |
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