Different air-water spray regulations affect the healing of Er,Cr:YSGG laser incisions

Autor: Felipe Fornias Sperandio, Daiane Thais Meneguzzo, Patricia Aparecida Ana, Suzana Cantanhede Orsini Machado de Sousa, Leila Soares Ferreira, Luciane Hiramatsu Azevedo
Rok vydání: 2010
Předmět:
Zdroj: Lasers in Medical Science. 26:257-265
ISSN: 1435-604X
0268-8921
DOI: 10.1007/s10103-010-0849-y
Popis: Surgeries performed with high-intensity laser devices may be improved with accurate protocols, including the air-water spray regulation. Thus, this study sought to investigate the healing process of wounds made on the dorsum of rat tongues using an Er,Cr:YSGG laser device with different air-water spray regulations. The incisions were made on the dorsum of Wistar rat tongues using an Er,Cr:YSGG laser with three different air-water spray regulations (100/0%, 50/50%, 11/7%). Scalpel incisions functioned as controls. The sacrifices occurred between 0 and 14 days after surgery. Morphological, histological, and immunohistochemical (fibronectin and type III collagen) analysis of the wounds were performed. The air-water spray regulation influenced wound healing and the inflammatory response, especially in the earlier stages. Incisions performed using the 100/0% air/water spray regulation had the worst results, expressing a greater amount of fibronectin and type III collagen. The 50/50% air/water spray regulation brought in a non-clear surgical field and poor laser interaction with the tissue. The 11/7% air/water spray regulation showed the best clinical results and less pronounced histological events. According to the results encountered, the air-water spray should be regulated to improve surgery.
Databáze: OpenAIRE
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