Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus
| Autor: | Eugene L. Brown, Joseph Wooters, Mary Collins, Rachel E. Meyers, Jonathan N. Kremsky, Joseph P. Dougherty |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1987 |
| Předmět: |
chemistry.chemical_classification
DNA ligase Aldehydes Oligonucleotide Carboxylic acid Nile red Carboxylic Acids DNA Biology Hydrazide Aldehyde Combinatorial chemistry chemistry.chemical_compound chemistry Biochemistry Oligodeoxyribonucleotides Polynucleotide Genetics Indicators and Reagents Chromatography High Pressure Liquid |
| Popis: | A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splint, a single stranded 98mer was efficiently joined to the immobilized synthetic fragment. After impregnation of the latex microspheres with the fluorescent dye, Nile Red and attachment of an aldehyde 16mer, 5 X 10(5) bead-DNA conjugates could be detected with a conventional fluorimeter. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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