Sex steroids modulate luteinizing hormone-releasing hormone secretion in a cholinergic cell line from the basal forebrain
| Autor: | Juan Ramón Martínez-Morales, Rafael Alonso, JG Hernandez-Jimenez, J. K. Blusztajn, I. López-Coviella, Ricardo Reyes, Guadalberto Hernández, A. R. Bello |
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| Rok vydání: | 2001 |
| Předmět: |
endocrine system
medicine.medical_specialty Biology Gonadotropic cell Progesterone receptor Cell Line Choline O-Acetyltransferase Gonadotropin-Releasing Hormone Mice Prosencephalon Choline acetyltransferase Internal medicine medicine Estrogen receptor Animals Estrogen Receptor beta RNA Messenger Cholinergic neuron Receptor Gonadal Steroid Hormones Neurons Basal forebrain Reverse Transcriptase Polymerase Chain Reaction General Neuroscience luteinizing hormone/choriogonadotropin receptor SN56 cells Estrogen Receptor alpha Septum Immunohistochemistry Acetylcholine eye diseases Electrophysiology Endocrinology Animals Newborn Receptors Estrogen Sex steroids Hormone receptor Cholinergic Luteinizing hormone Receptors Progesterone |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0306-4522 |
| Popis: | 7 páginas, 3 figuras, 1 tabla. The function of a particular neuronal population is in part determined by its neurotransmitter phenotype. We have found that a neuronal-derived septal cell line (SN56), known for its cholinergic properties, also synthesizes and releases luteinizing hormone-releasing hormone. In addition, these cells express the messenger RNAs encoding estrogen and progesterone receptors. The activation of these receptors by their respective ligands cooperatively modulates the depolarization-induced release of luteinizing hormone-releasing hormone in these cells. We have also found that a number of septal neurons in postnatal (1-week-old) mice are immunoreactive to both choline acetyltransferase and luteinizing hormone-releasing hormone. These results indicate that both neurotransmitters, acetylcholine and luteinizing hormone-releasing hormone, may co-exist in septal neurons of the CNS and that they could be modulated by gonadal hormones, and suggest that luteinizing hormone-releasing hormone could be involved in some of the actions of sex steroids on cholinergic neurotransmission. Supported by grants PB97-1472-C03-01 (DGESIC, Spain) and 1FD97-1065-C03-01 (European Commission) to R.A., PB97-1472-C03-02 (DGESIC, Spain) to A.R.B., and AG09525 (NIH, USA) to J.K.B. Partially supported by Lilly S.A., Astra-Zeneca, ITC, and CEPSA. JG H-J and JR M-M held research fellowships from DGESIC and Lilly S.A., respectively. |
| Databáze: | OpenAIRE |
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