Mesorhizobium delmotii and Mesorhizobium prunaredense are two new species containing rhizobial strains within the symbiovar anthyllidis

Autor: Brigitte Brunel, Maurine Bonabaud, Emeric Dubois, Géraldine Maynaud, Jean Claude Cleyet-Marel, Anne Willems, Roba Mohamad, Antoine Le Quéré, Marjorie Pervent
Přispěvatelé: Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratory of Microbiology, Dept. Biochemistry and Microbiology, Universiteit Gent = Ghent University [Belgium] (UGENT), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), Ghent University [Belgium] (UGENT), ANR-10-INBS-09
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Sequence analysis
Anthyllis vulneraria
nodosité racinaire
Biology
Applied Microbiology and Biotechnology
Microbiology
légumineuse
DNA sequencing
03 medical and health sciences
RNA
Ribosomal
16S

Symbiosis
Gene
Ecology
Evolution
Behavior and Systematics

Phylogeny
Base Composition
Strain (chemistry)
Mass spectrometry
mesorhizobium
Mesorhizobium
Fabaceae
Sequence Analysis
DNA

Average nucleotide identity
legume
16S ribosomal RNA
biology.organism_classification
root
symbiosis
Housekeeping gene
anthyllis
racine
030104 developmental biology
spectrométrie de masse à ionisation secondaire
séquençage du génome
root nodules
Multilocus sequence analysis
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
Root Nodules
Plant

symbiose
Genome
Bacterial

Multilocus Sequence Typing
Rhizobium
Zdroj: Systematic and Applied Microbiology
Systematic and Applied Microbiology, Elsevier, 2017, 40 (3), pp.135-143. ⟨10.1016/j.syapm.2017.01.004⟩
ISSN: 0723-2020
DOI: 10.1016/j.syapm.2017.01.004⟩
Popis: Eight mesorhizobial symbiotic strains isolated from Anthyllis vulneraria root-nodules were studied and compared taxonomically with defined Mesorhizobium species. All strains presented identical 165 rDNA sequences but can be differentiated by multilocus sequence analysis of housekeeping genes (recA, atpD, glnll and dnaK). Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analyses separate these strains in two groups and a separate strain. Levels of DNA-DNA relatedness were less than 55% between representative strains and their closest Mesorhizobium reference relatives. The two groups containing four and three strains, respectively, originating from border mine and non-mining areas in Cevennes, were further phenotypically characterized. Groupings were further supported by average nucleotide identity values based on genome sequencing, which ranged from 80 to 92% with their close relatives and with each other, confirming these groups represent new Mesorhizobium species. Therefore, two novel species Mesorhizobium delmotii sp. nov. (type strain STM4623(T) = LMG 296401(T)= CFBP 84361(T) ) and Mesorhizobium prunaredense sp. nov. (type strain STM4891(T) = LMG 29641(T) = CFBP 8437(T)) are proposed. Type strains of the two proposed species share accessory common nodulation genes within the new symbiovar anthyllidis as found in the Mesorhizobium metallidurans type strain.
Databáze: OpenAIRE