IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers
Autor: | Karelia Velázquez, Miguel A. Perez-Amador, Manuel Talon, Francisco R. Tadeo, Javier Terol, José Guerri, Susana Ruiz-Ruiz, Daniel Ventimilla, Mª. Carmen Vives |
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Rok vydání: | 2021 |
Předmět: |
Somatic embryogenesis
Cell separation Nicotiana benthamiana Flowers Plant Science Cell wall Abscission Hormone peptide Gene Expression Regulation Plant Arabidopsis Tobacco Abscission signaling module Arabidopsis thaliana Amino Acid Sequence F63 Plant physiology - Reproduction H20 Plant diseases Solanaceae Plant Proteins biology Research Abscission Zone fungi Botany food and beverages Plants Genetically Modified biology.organism_classification F60 Plant physiology and biochemistry Cell biology Gene silencing and overexpression Inflorescence QK1-989 Abscission zone LRR-RLKs Sequence Alignment Signal Transduction |
Zdroj: | electronico ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias instname ReDivia: Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias Instituto Valenciano de Investigaciones Agrarias (IVIA) Digital.CSIC. Repositorio Institucional del CSIC BMC Plant Biology, Vol 21, Iss 1, Pp 1-14 (2021) BMC Plant Biology |
Popis: | Background Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at specific sites on the plant body known as abscission zones. In Arabidopsis thaliana, abscission of floral organs and cauline leaves is regulated by the interaction of the hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), a pair of redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE2 (HSL2), and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, the functionality of this abscission signaling module has not yet been demonstrated in other plant species. Results The expression of the pair of NbenIDA1 homeologs and the receptor NbenHAE.1 was supressed at the base of the corolla tube by the inoculation of two virus-induced gene silencing (VIGS) constructs in Nicotiana benthamiana. These gene suppression events arrested corolla abscission but did not produce any obvious effect on plant growth. VIGS plants retained a higher number of corollas attached to the flowers than control plants, an observation related to a greater corolla breakstrength. The arrest of corolla abscission was associated with the preservation of the parenchyma tissue at the base of the corolla tube that, in contrast, was virtually collapsed in normal corollas. In contrast, the inoculation of a viral vector construct that increased the expression of NbenIDA1A at the base of the corolla tube negatively affected the growth of the inoculated plants accelerating the timing of both corolla senescence and abscission. However, the heterologous ectopic overexpression of citrus CitIDA3 and Arabidopsis AtIDA in N. benthamiana did not alter the standard plant phenotype suggesting that the proteolytic processing machinery was unable to yield active peptides. Conclusion Here, we demonstrate that the pair of NbenIDA1 homeologs encoding small peptides of the IDA-like family and the receptor NbenHAE.1 control cellular breakdown at the base of the corolla tube awhere an adventitious AZ should be formed and, therefore, corolla abscission in N. benthamiana flowers. Altogether, our results provide the first evidence supporting the notion that the IDA-HAE/HSL2 signaling module is conserved in angiosperms. |
Databáze: | OpenAIRE |
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