Simultaneous electrochemical detection of multiple analytes based on dual signal amplification of single-walled carbon nanotubes and multi-labeled graphene sheets
| Autor: | Yan Wang, Yali Yuan, Ruo Yuan, Ying Zhuo, Yaqin Chai, Lijuan Bai |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Aptamer Biophysics Bioengineering Nanotechnology Biosensing Techniques Carbon nanotube Platinum nanoparticles Nanocomposites law.invention Biomaterials Limit of Detection law Humans Glucose oxidase Platinum Platelet-Derived Growth Factor Detection limit biology Nanotubes Carbon Graphene Thrombin Electrochemical Techniques Aptamers Nucleotide Chemical engineering Mechanics of Materials Colloidal gold Ceramics and Composites biology.protein Graphite Gold Biosensor |
| Zdroj: | Biomaterials. 33:1090-1096 |
| ISSN: | 0142-9612 |
| Popis: | In this work, a sandwich-type electrochemical aptasensor for simultaneous sensitive detection of platelet-derived growth factor (PDGF) and thrombin is fabricated. Reduced graphene oxide sheets (rGS) are used as matrices to immobilize the redox probes, which are subsequently coated with platinum nanoparticles (PtNPs) to form the PtNPs-redox probes-rGS nanocomposites. With the employment of the as prepared nanocomposites, a signal amplification strategy was described based on bienzyme (glucose oxidase and horseradish peroxidase) modified PtNPs-redox probes-rGS nanocomposites as the tracer labels for secondary aptamers (Apt II) through sandwiched assay. Gold nanoparticles functionalized single-walled carbon nanotubes (AuNPs@SWCNTs) as the biosensor platform enhance the surface area to capture a large amount of primary aptamers (Apt I), thus amplifying the detection response. The experiment results show that the multi-labeled PtNPs-redox probes-rGS nanocomposites display satisfying electrochemical redox activity and highly electrocatalytic activity of PtNPs and bienzyme, which exhibit high sensitivity for detection of proteins. The linear range of PDGF is 0.01-35 nM with a detection limit of 8 pM, while the linear ranges from 0.02 to 45 nM and a detection limit of 11 pM for thrombin are obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect