Characterization of blue light irradiation effects on pathogenic and nonpathogenicEscherichia coli
| Autor: | Courtney M. Abana, Kirsten R. Guckes, Maria Hadjifrangiskou, Hubaida Fuseini, Jennifer G. Powers, Bridget R. Rogers, John R. Brannon, Rebecca A. Ebbott, Taryn L. Dunigan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Light 030106 microbiology Colony Count Microbial Bacterial growth Biology medicine.disease_cause Microbiology 03 medical and health sciences Adenosine Triphosphate Escherichia coli medicine persisters Original Research Blue light Gastrointestinal tract Microbial Viability Phylogenetic tree Strain (chemistry) pathogenesis E. coli Antimicrobial biology.organism_classification 030104 developmental biology membranes Bacteria |
| Zdroj: | MicrobiologyOpen |
| ISSN: | 2045-8827 |
| DOI: | 10.1002/mbo3.466 |
| Popis: | Blue light irradiation (BLI) is an FDA‐approved method for treating certain types of infections, like acne, and is becoming increasingly attractive as an antimicrobial strategy as the prevalence of antibiotic‐resistant “superbugs” rises. However, no study has delineated the effectiveness of BLI throughout different bacterial growth phases, especially in more BLI‐tolerant organisms such as Escherichia coli. While the vast majority of E. coli strains are nonpathogenic, several E. coli pathotypes exist that cause infection within and outside the gastrointestinal tract. Here, we compared the response of E. coli strains from five phylogenetic groups to BLI with a 455 nm wavelength (BLI 455), using colony‐forming unit and ATP measurement assays. Our results revealed that BLI 455 is not bactericidal, but can retard E. coli growth in a manner that is dependent on culture age and strain background. This observation is critical, given that bacteria on and within mammalian hosts are found in different phases of growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text