LARP-1 promotes oogenesis by repressingfem-3in theC. elegansgermline
| Autor: | Esther Zanin, Anne Pacquelet, Rafal Ciosk, Monica Gotta, Claudia Scheckel |
|---|---|
| Přispěvatelé: | De Villemeur, Hervé, Institute of Biochemistry, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Ludwig Institute for Cancer Research, University of California [San Diego] (UC San Diego), University of California (UC)-University of California (UC), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Friedrich Miescher Institute for Biomedical Research (FMI), Novartis Research Foundation, Department of Genetic Medicine and Development, University of Geneva School of Medicine, University of California-University of California, Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES) |
| Rok vydání: | 2010 |
| Předmět: |
Male
Protein family MAP Kinase Signaling System Caenorhabditis elegans Proteins/genetics/*metabolism RNA-binding protein [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Mitogen-Activated Protein Kinase 1/genetics/metabolism Spermatozoa/physiology Oogenesis Germline 03 medical and health sciences 0302 clinical medicine Sex Determination Processes/*physiology [SDV.BDD] Life Sciences [q-bio]/Development Biology Animals Caenorhabditis elegans Caenorhabditis elegans Proteins ddc:612 [SDV.BC] Life Sciences [q-bio]/Cellular Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology Transcription factor Phylogeny [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology 030304 developmental biology Mitogen-Activated Protein Kinase 1 Genetics 0303 health sciences Oogenesis/genetics/*physiology RNA-Binding Proteins [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Cell Biology Sex Determination Processes biology.organism_classification Spermatozoa Ubiquitin ligase Caenorhabditis elegans/genetics/metabolism/*physiology Mutation Oocytes biology.protein Female RNA-Binding Proteins/*metabolism Oocytes/physiology 030217 neurology & neurosurgery Cullin |
| Zdroj: | Journal of Cell Science Journal of Cell Science, 2010, 123 (Pt 16), pp.2717-24. ⟨10.1242/jcs.066761⟩ Journal of Cell Science, Company of Biologists, 2010, 123 (Pt 16), pp.2717-24. ⟨10.1242/jcs.066761⟩ Journal of Cell Science, Vol. 123, No Pt 16 (2010) pp. 2717-2724 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.066761 |
| Popis: | International audience; LA-related protein 1 (LARP-1) belongs to an RNA-binding protein family containing a LA motif. Here, we identify LARP-1 as a regulator of sex determination. In C. elegans hermaphrodites, a complex regulatory network regulates the switch from sperm to oocyte production. We find that simultaneous depletion of larp-1 and the Nanos homologue nos-3 results in germline masculinization. This phenotype is accompanied by a strong reduction of the levels of TRA-1, a GLI-family transcription factor that promotes oogenesis. TRA-1 levels are regulated by CBC(FEM-1), a ubiquitin ligase consisting of the FEM proteins, FEM-1, FEM-2 and FEM-3 and the cullin CUL-2. We show that both the masculinization phenotype and the reduction of TRA-1 levels observed in nos-3;larp-1 mutants require fem-3 activity, suggesting that nos-3 and larp-1 regulate the sperm-oocyte switch by inhibiting the fem genes. Consistently, fem-3 mRNA levels are increased in larp-1 mutants. By contrast, levels of fem-3 mRNA are not affected in nos-3 mutants. Therefore, our data indicate that LARP-1 and NOS-3 promote oogenesis by regulating fem-3 expression through distinct mechanisms. |
| Databáze: | OpenAIRE |
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