SCDrs41290540 single‐nucleotide polymorphism modifies miR‐498 binding and is associated with a decreased risk of coronary artery disease

Autor: Xing‐dong Xiong, Xiaojian Yin, Zhou Liu, Hui Mai, Jiajing Lin, Shouchao Wei, Wanjuan Peng, Keshen Li, Hua Tao, Li Hu, Guangning Li, Kanglan Li, Zhijun Lin, Haihong Zhou, Feng Yao, Wanxin Jie
Rok vydání: 2020
Předmět:
Blood Glucose
Male
0301 basic medicine
Untranslated region
congenital
hereditary
and neonatal diseases and abnormalities
medicine.medical_specialty
lcsh:QH426-470
single‐nucleotide polymorphism
Blood Pressure
Single-nucleotide polymorphism
Coronary Artery Disease
stearoyl‐CoA desaturase
030105 genetics & heredity
Polymorphism
Single Nucleotide
Coronary artery disease
03 medical and health sciences
Western blot
hemic and lymphatic diseases
Internal medicine
Genotype
Genetics
Humans
Medicine
SNP
Luciferase
cardiovascular diseases
Molecular Biology
Cells
Cultured
Genetics (clinical)
Aged
microRNA
medicine.diagnostic_test
business.industry
Three prime untranslated region
Original Articles
Middle Aged
medicine.disease
Lipids
lcsh:Genetics
MicroRNAs
HEK293 Cells
030104 developmental biology
Endocrinology
Female
Original Article
business
3′‐untranslated region
Stearoyl-CoA Desaturase
Zdroj: Molecular Genetics & Genomic Medicine
Molecular Genetics & Genomic Medicine, Vol 8, Iss 3, Pp n/a-n/a (2020)
ISSN: 2324-9269
DOI: 10.1002/mgg3.1136
Popis: Background Atherosclerosis is the primary cause of coronary artery disease (CAD), and stearoyl‐CoA desaturase (SCD) is associated with atherosclerosis. However, the associations between variants of SCD and CAD have not yet been decided. Methods This study analyzed SCD rs41290540 single‐nucleotide polymorphism (SNP) in the 3′‐untranslated region for an association with a risk of CAD among the Chinese Han population. CAD patients and controls were genotyped for SNP rs41290540 in SCD by SNaPshot. The binding affinity of miR‐498 to rs41290540 was determined by a luciferase assay, and SCD expression was assessed using Western blot. Results A total of 969 CAD patients and 1,095 control subjects were involved in this study. The SCD rs41290540CC genotype is associated with a decreased risk of CAD compared with the AA genotype. Furthermore, the CC genotype is associated with lower serum total cholesterol (TC). Western blot analysis demonstrated that miR‐498 suppressed the expression of SCD. A luciferase assay confirmed that rs41290540 A>C variation in the SCD 3′UTR inhibits miR‐498 binding. Conclusion This study demonstrates that the SCD rs41290540 may be associated with a decreased risk of CAD, lower serum TC, and decreased miR‐498 binding.
We find that the stearoyl‐CoA desaturase rs41290540 may reduce the risk of coronary artery disease. Furthermore, we prove that rs41290540 works by reducing miR‐498 binding.
Databáze: OpenAIRE
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