Upregulation of discoidin domain receptor 2 in nasopharyngeal carcinoma

Autor: Ching-Hwa Tsai, You Chang Lo, Ying Piao Wang, Huey Huey Chua, Tzung Shiahn Sheen, Te-Huei Yeh, Ya Ching Chou, Yu Tzu Huang
Rok vydání: 2008
Předmět:
Zdroj: Head & Neck. 30:427-436
ISSN: 1097-0347
1043-3074
DOI: 10.1002/hed.20724
Popis: Background. Nasopharyngeal carcinoma (NPC) is associated with Epstein-Barr virus (EBV) and has high meta- static potential. Discoidin domain receptors (DDR1, DDR2) are receptor-type tyrosine kinases activated by collagen. Their abil- ity to induce expression of matrix metalloproteinase is related with tumor invasion. Therefore, we aim to investigate DDRs gene expression and its regulation in NPC. Methods and Results. By use of real-time quantitative poly- merase chain reaction (Q-PCR), DDR2 gene expression but not DDR1 was significantly higher in primary and metastatic NPC. DDR2 was predominantly distributed in NPC tumor cells rather than in infiltrating lymphocytes. EBV Z-transactivator (Zta) trans- fection may distinctly elevate DDR2 level. Furthermore, data from reporter assay indicate that Zta could transactivate DDR2 pro- moter activity, suggesting the possible upregulation mechanism. Conclusion. DDR2 was differentially upregulated in NPC and modulated by EBV Zta protein. DDR2 may play a role in NPC invasion and serve as a diagnostic and therapeutic target.
Databáze: OpenAIRE
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