Effects of ammonioalkyl sulfonate internal salts on electrokinetic micropump performance and reversed-phase high-performance liquid chromatographic separations
| Autor: | Brian Kirby, David S. Reichmuth |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Aqueous solution Chromatography Molar concentration Organic Chemistry Analytical chemistry Micropump General Medicine Reversed-phase chromatography Biochemistry Streaming current Analytical Chemistry Quaternary Ammonium Compounds chemistry.chemical_compound Electrokinetic phenomena Sulfonate chemistry Salts Chromatography High Pressure Liquid Alkyl |
| Zdroj: | Journal of Chromatography A. 1013:93-101 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(03)01468-7 |
| Popis: | Ammonioalkyl sulfonate internal salts are explored owing to their potential for improving electrokinetic pumps used to perform miniaturized HPLC separations. The internal salts investigated can be added at high molarity since they are net-neutral, and furthermore show potential for increasing electroosmotic pumping owing to their large positive dielectric increment. Streaming potential measurements of buffered aqueous systems with varying concentrations of ammonioalkyl sulfonate internal salts have been used to measure these dielectric increments, which increase with the length of the alkyl linker. Due to their positive dielectric increments and their decremental effect on solution conductivity, all of the measured species are predicted to improve the pressure generation (up to 85%) and efficiency performance (up to 140%) of electrokinetic pumps when added at 1 M concentration. RP-HPLC separations with an ammonioalkyl sulfonate (TMAPS) have been performed and indicate that separation performance is essentially unaffected by these species. These results indicate the potential for a variety of ammonioalkyl sulfonates to be used to improve electrokinetic pump performance for miniaturized HPLC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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