Determination of reference intervals for common chemistry and immunoassay tests for Kenyan adults based on an internationally harmonized protocol and up-to-date statistical methods

Autor: Jane Mwangi, P. J. Ojwang, Caroline Wambua, Angela Amayo, Rajiv T Erasmus, Zul Premji, Elizabeth Kagotho, Daniel Maina, Kiyoshi Ichihara, Mariza Hoffman, Geoffrey Omuse, Alice Kanyua
Rok vydání: 2020
Předmět:
Male
Biochemistry
chemistry.chemical_compound
Spectrum Analysis Techniques
Endocrinology
Reference Values
Medicine and Health Sciences
Urea
Ethnicities
Thyroid
Immunoassay
education.field_of_study
Multidisciplinary
medicine.diagnostic_test
Organic Compounds
Anemia
Hematology
Middle Aged
Reference Standards
Lipids
Healthy Volunteers
Chemistry
Cholesterol
Spectrophotometry
Data Interpretation
Statistical
Physical Sciences
Medicine
Female
Anatomy
Research Article
Adult
medicine.medical_specialty
Adolescent
Endocrine Disorders
Science
Population
Endocrine System
Research and Analysis Methods
Young Adult
Sex Factors
Internal medicine
Diabetes Mellitus
medicine
Humans
Immunoassays
education
Aged
African People
Protocol (science)
Creatinine
Transferrin saturation
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
medicine.disease
Kenya
Reference intervals
Biological Variation
Population
chemistry
Metabolic Disorders
People and Places
Immunologic Techniques
Population Groupings
Biomarkers
Blood Chemical Analysis
TBIL
Densitometry
Turbidimetry
Zdroj: PLoS ONE, Vol 15, Iss 7, p e0235234 (2020)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0235234
Popis: BackgroundDue to a lack of reliable reference intervals (RIs) for Kenya, we set out to determine RIs for 40 common chemistry and immunoassay tests as part of the IFCC global RI project.MethodsApparently healthy adults aged 18-65 years were recruited according to a harmonized protocol and samples analyzed using Beckman-Coulter analyzers. Value assigned serum panels were measured to standardize chemistry results. The need for partitioning reference values by sex and age was based on between-subgroup differences expressed as standard deviation ratio (SDR) or bias in lower or upper limits (LLs and ULs) of the RI. RIs were derived using a parametric method with/without latent abnormal value exclusion (LAVE).ResultsSex-specific RIs were required for uric acid, creatinine, total bilirubin (TBil), total cholesterol (TC), ALT, AST, CK, GGT, transferrin, transferrin saturation (TfSat) and immunoglobulin-M. Age-specific RIs were required for glucose and triglyceride for both sexes, and for urea, magnesium, TC, HDL-cholesterol ratio, ALP, and ferritin for females. LAVE was effective in optimizing RIs for AST, ALT, GGT iron-markers and CRP by reducing influence of latent anemia and metabolic diseases. Thyroid profile RIs were derived after excluding volunteers with anti-thyroid antibodies. Kenyan RIs were comparable to those of other countries participating in the global study with a few exceptions such as higher ULs for TBil and CRP.ConclusionsKenyan RIs for major analytes were established using harmonized protocol from well-defined reference individuals. Standardized RIs for chemistry analytes can be shared across sub-Saharan African laboratories with similar ethnic and life-style profile.
Databáze: OpenAIRE
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