Recent Mobility of Casposons, Self-Synthesizing Transposons at the Origin of the CRISPR-Cas Immunity
| Autor: | Mart Krupovic, Patrick Forterre, Kira S. Makarova, Sergey Shmakov, Eugene V. Koonin |
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| Přispěvatelé: | Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE), Institut Pasteur [Paris] (IP), Skolkovo Institute of Science and Technology [Moscow] (Skoltech), National Center for Biotechnology Information (NCBI), P.F. was supported by the European Union's Seventh Framework Program (FP/2007-2013)/Project EVOMOBIL - ERC Grant Agreement no. 340440. E.V.K. and K.S.M. are supported by intramural funds of the US Department of Health and Human Services (to the National Library of Medicine)., European Project: 340440,EC:FP7:ERC,ERC-2013-ADG,EVOMOBIL(2014), Institut Pasteur [Paris] |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transposable element MESH: Genome Archaeal Gene Transfer Horizontal Inverted repeat Archaeal Proteins 030106 microbiology Molecular Sequence Data Computational biology Biology MESH: Base Sequence Genome 03 medical and health sciences Phylogenetics Genome Archaeal casposons [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Genetics Recombinase transposition CRISPR Clustered Regularly Interspaced Short Palindromic Repeats MESH: Endodeoxyribonucleases CRISPR-Cas MESH: Phylogeny Ecology Evolution Behavior and Systematics Phylogeny Endodeoxyribonucleases MESH: Molecular Sequence Data Base Sequence mobile genetic elements MESH: Archaeal Proteins [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Gene Transfer Horizontal MESH: DNA Transposable Elements MESH: Clustered Regularly Interspaced Short Palindromic Repeats Horizontal gene transfer DNA Transposable Elements Methanosarcina barkeri Mobile genetic elements self-synthesizing transposons MESH: Methanosarcina barkeri Research Article |
| Zdroj: | Genome Biology and Evolution Genome Biology and Evolution, 2016, 8 (2), pp.375-86. ⟨10.1093/gbe/evw006⟩ Genome Biology and Evolution, Society for Molecular Biology and Evolution, 2016, 8 (2), pp.375-86. ⟨10.1093/gbe/evw006⟩ |
| ISSN: | 1759-6653 |
| DOI: | 10.1093/gbe/evw006⟩ |
| Popis: | International audience; Casposons are a superfamily of putative self-synthesizing transposable elements that are predicted to employ a homolog of Cas1 protein as a recombinase and could have contributed to the origin of the CRISPR-Cas adaptive immunity systems in archaea and bacteria. Casposons remain uncharacterized experimentally, except for the recent demonstration of the integrase activity of the Cas1 homolog, and given their relative rarity in archaea and bacteria, original comparative genomic analysis has not provided direct indications of their mobility. Here, we report evidence of casposon mobility obtained by comparison of the genomes of 62 strains of the archaeon Methanosarcina mazei. In these genomes, casposons are variably inserted in three distinct sites indicative of multiple, recent gains, and losses. Some casposons are inserted into other mobile genetic elements that might provide vehicles for horizontal transfer of the casposons. Additionally, many M. mazei genomes contain previously undetected solo terminal inverted repeats that apparently are derived from casposons and could resemble intermediates in CRISPR evolution. We further demonstrate the sequence specificity of casposon insertion and note clear parallels with the adaptation mechanism of CRISPR-Cas. Finally, besides identifying additional representatives in each of the three originally defined families, we describe a new, fourth, family of casposons. |
| Databáze: | OpenAIRE |
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