A Promising Serum Autoantibody Marker, Anti-Heat Shock Protein 90α, for Cholangiocarcinoma
| Autor: | Panaratana Ratanasuwan, Zhiliang Wu, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Amornrat Juasook, Watcharin Loilome, Atit Silsirivanit, Thidarut Boonmars, Pranee Sriraj |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Epidemiology Blotting Western Molecular Sequence Data information science Hamster Enzyme-Linked Immunosorbent Assay Opisthorchiasis Cholangiocarcinoma Western blot Cricetinae Heat shock protein parasitic diseases Biomarkers Tumor medicine Animals Humans Immunoprecipitation Amino Acid Sequence HSP90 Heat-Shock Proteins cardiovascular diseases Autoantibodies Gel electrophoresis biology medicine.diagnostic_test Opisthorchis fungi Public Health Environmental and Occupational Health Autoantibody medicine.disease Molecular biology Bile Ducts Intrahepatic Bile Duct Neoplasms ROC Curve Oncology Case-Control Studies cardiovascular system biology.protein Biomarker (medicine) Antibody |
| Zdroj: | Asian Pacific Journal of Cancer Prevention. 16:5779-5785 |
| ISSN: | 1513-7368 |
| Popis: | The present study was designed to investigate cholangiocarcinoma (CCA) antibodies in hamster serum. Hamster CCA cell lines were processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A candidate biomarker was confirmed by immunoprecipitation and western blot, and was further analyzed using ELISA and sera from normal control hamsters, hamsters with opisthorchiasis and hamsters with various stages of CCA, as well as from CCA patients and healthy individuals. One candidate marker was identified as HSP90α, as indicated by a high level of anti-HSP90α in hamster CCA sera. It was found that the levels of anti-HSP90α were specifically elevated in the sera of hamsters with CCA compared with other groups and progressively increased with the clinical stage. At the cut-off point of 0.4850 on the receiver operating characteristic curve, anti-HSP90α could discriminate CCA from healthy control groups with a sensitivity of 76.2%, specificity of 71.4% and total accuracy 75.5%. In the present study, we have shown that anti-HSP90α may be a potential useful serum biomarker to discriminate CCA cases from healthy persons. |
| Databáze: | OpenAIRE |
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