A vinyl sulfone clicked carbon dot-engineered microfluidic paper-based analytical device for fluorometric determination of biothiols
| Autor: | Mariano Ortega-Muñoz, Fernando Hernandez-Mateo, Inmaculada Ortiz-Gomez, Francisco Santoyo-Gonzalez, Alfonso Salinas-Castillo, Luis Fermín Capitán-Vallvey, Antonio Marin-Sanchez, Ignacio de Orbe-Payá |
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| Rok vydání: | 2020 |
| Předmět: |
Paper
Microfluidics Nanochemistry 02 engineering and technology 010402 general chemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Lab-On-A-Chip Devices Quantum Dots Humans Cysteine Sulfones Cellulose Homocysteine Detection limit Nanocomposite Chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Glutathione Fluorescence Combinatorial chemistry Carbon 0104 chemical sciences Spectrometry Fluorescence Covalent bond Reagent Click Chemistry 0210 nano-technology |
| Zdroj: | Microchimica Acta. 187 |
| ISSN: | 1436-5073 0026-3672 |
| Popis: | A microfluidic paper-based analytical device integrating carbon dot (CDs) is fabricated and used for a fluorometric off-on assay of biothiols. Vinyl sulfone (VS) click immobilization of carbon dots (CDs) on paper was accomplished by a one-pot simplified protocol that uses divinyl sulfone (DVS) as a homobifunctional reagent. This reagent mediated both the click oxa-Michael addition to the hydroxyl groups of cellulose and ulterior covalent grafting of the resulting VS paper to NH2-functionalized CDs by means of click aza-Michael addition. The resulting cellulose nanocomposite was used to engineer an inexpensive and robust microfluidic paper-based analytical device (μPAD) that is used for a reaction-based off-on fluorometric assay of biothiols (GSH, Cys, and Hcy). The intrinsic blue fluorescence of CDs (with excitation/emission maxima at 365/450 nm) is turned off via the heavy atom effect of an introduced iodo group. Fluorescence is turned on again due to the displacement of iodine by reaction with a biothiol. The increase in fluorescence is related to the concentration over a wide range (1 to 200 μM for GSH and 5–200 μM for Cys and Hcy, respectively), and the assay exhibits a low detection limit (0.3 μM for GSH and Cys and 0.4 μM for Hcy). The method allows for rapid screening and can also be used in combination with a digital camera readout. |
| Databáze: | OpenAIRE |
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