Correlation of the release of amines and antagonists with their effects
| Autor: | B.J. Key, R.J. Boakes, J.M. Candy, Eve Worton |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Biogenic Amines
Chlorpromazine Stereochemistry medicine.medical_treatment Brain tissue In Vitro Techniques Sulfur Radioisotopes Methylation Biogenic Monoamines Fluorescence Diffusion Norepinephrine Cellular and Molecular Neuroscience medicine Animals Carbon Radioisotopes Saline Brain Chemistry Neurons Radioisotopes Pharmacology Chemistry Brain Electroencephalography Iontophoresis Molecular Weight Kinetics Cats Biophysics Autoradiography Regression Analysis Sodium Isotopes |
| Zdroj: | Neuropharmacology. 13:423-430 |
| ISSN: | 0028-3908 |
| DOI: | 10.1016/0028-3908(74)90130-0 |
| Popis: | The microiontophoretic release of [14C]-noradrenaline into saline and brain tissue and the effect of a backing current of 15 nA on this release has been determined. It was found that the transport number of noradrenaline was similar for saline and tissue and that the backing current could markedly reduce the release of noradrenaline. The microiontophoretic release of [35S]-chlorpromazine has been studied. The release was linearly related to the charge passed for only two out of the ten micropipettes tested and the transport number for these was very low. An attempt has been made to determine the range of diffusion after microiontophoretic release of α-methylnoradrenaline and noradrenaline using the fluorescence method for the demonstration of biogenic monoamines and soluble compound autoradiography. The results show that these substances can diffuse for considerable distances and therefore possibly affect more than one neurone. There appears to be a correlation between the effects of perfusing a range of concentrations of noradrenaline on the EEG and the amount of noradrenaline that is taken up by the tissue. The amount of noradrenaline that diffuses into the tissue under the conditions that produce phasic arousal can be released microiontophoretically by a relatively low charge. It may therefore be possible to use microiontophoresis to study the mechanisms underlying more gross physiological changes. |
| Databáze: | OpenAIRE |
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