Occurrence of Marek’s disease in Poland on the basis of diagnostic examination in 2015–2018
| Autor: | Karolina Piekarska, Agnieszka Stolarek, Jowita Samanta Niczyporuk, Hanna Czekaj, Wojciech Kozdruń, Natalia Styś-Fijoł |
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| Rok vydání: | 2020 |
| Předmět: |
animal structures
040301 veterinary sciences viruses Veterinary medicine animal diseases Disease Biology marek’s disease Virus 0403 veterinary science 03 medical and health sciences immune system diseases hemic and lymphatic diseases SF600-1100 real-time pcr 030304 developmental biology 0303 health sciences Marek's disease General Veterinary poultry Broiler 04 agricultural and veterinary sciences biology.organism_classification Virology Real-time polymerase chain reaction Flock Research Article |
| Zdroj: | Journal of Veterinary Research, Vol 64, Iss 4, Pp 503-507 (2020) Journal of Veterinary Research |
| ISSN: | 2450-8608 |
| DOI: | 10.2478/jvetres-2020-0079 |
| Popis: | Introduction Marek’s disease (MD) is a tumourous disease caused by Marek’s disease virus (MDV) and most commonly described in poultry. The aim of the study was to determine the occurrence of Marek’s disease virus infections in Poland and analyse clinical cases in the years 2015–2018. Material and Methods The birds for diagnostic examination originated from 71 poultry flocks of various types of production. Birds were subjected to anatomopathological examination post mortem, during which liver and spleen sections and other pathologically changed internal organs were taken. These sections were homogenised with generally accepted methods, then total DNA was isolated and amplified with a real-time PCR. A pair of primers complementary to the MDV genome region encoding the meq gene were used. Results MDV infection was found predominantly in broiler chicken flocks (69.01%), and also in layer breeder (9.85%) and commercial layer flocks (7.04% each). Conclusion The results of research conducted in the years 2015–2018 clearly indicate that the problem of MDV infections is still current. |
| Databáze: | OpenAIRE |
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