In-111 Pseudomonas aeruginosa: A simple method of labeling live bacteria with a gamma-emitting radioisotope
| Autor: | Rex B. Shafer, Melody O'Connor Allen, Lee Forstrom, Kristine Bettin, Dale N. Gerding |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Time Factors
medicine.disease_cause Indium Microbiology Dogs Organometallic Compounds medicine Animals Radiology Nuclear Medicine and imaging Radioisotopes biology Pseudomonas aeruginosa Chemistry Stem Cells Pseudomonas Radiochemistry General Medicine Oxyquinoline biology.organism_classification Culture Media Blood Pseudomonadales Hydroxyquinolines Bacteria Pseudomonadaceae |
| Zdroj: | European Journal of Nuclear Medicine. 12:277-279 |
| ISSN: | 1619-7089 0340-6997 |
| DOI: | 10.1007/bf00251987 |
| Popis: | We describe a simple and reliable technique for labeling Pseudomonas aeruginosa with a readily available commercial preparation of indium-111 (111In) oxine. Labeling of a heavy bacterial suspension with 500 mu Ci of commercially prepared 111In-oxine resulted in a yield of 0.0004 mu Ci of cell-associated 111In per 10(6) colony-forming units (CFU). The label was 88% bacterially associated and did not effect viability of the organism. Radiolabeling a gram-negative organism with 111In-oxine provides a non-toxic, stable gamma-emitting bacterial tracer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink