Atypical E-cadherin expression in cell clusters overlying focally disrupted mammary myoepithelial cell layers: Implications for tumor cell motility and invasion
| Autor: | Morvarid Yousefi, Jinsong Ni, Chunling Gao, Shahreyar Shar Hashemi, Yan-gao Man, Jeffrey T. Mason, Xichen Zhang, Wan Wang, Joy Sheng |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Gene Expression Motility Breast Neoplasms Biology Pathology and Forensic Medicine Stroma Cell Movement medicine Humans Neoplasm Invasiveness Gene Muscle Cells Reverse Transcriptase Polymerase Chain Reaction Cadherin Carcinoma Ductal Breast Myoepithelial cell Epithelial Cells Cell Biology Cadherins Cell biology medicine.anatomical_structure Cytoplasm Female Duct (anatomy) Carcinoma in Situ |
| Zdroj: | Pathology - Research and Practice. 205:375-385 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2008.08.009 |
| Popis: | Our recent studies showed that cell clusters overlying focal myoepithelial cell layer disruptions (FMCLD) had a significantly higher rate of ER negativity, genetic instabilities, and expression of invasion-related genes than adjacent cells within the same duct. This study attempted to determine if these cells would show aberrant E-cadherin expression, which imparts greater propensity for cell motility and invasion. Consecutive sections from breast tumors with a high frequency of FMCLD were double-immunostained for E-cadherin and a panel of related markers. The E-cadherin mRNA levels in cells overlying FMCLD and adjacent cells within the same duct were compared using real-time PCR. Nearly all the cell clusters overlying FMCLD were strongly immunoreactive for E-cadherin, whereas their adjacent counterparts within the same duct were largely negative. Cell clusters overlying FMCLD were generally arranged as tongue-like projections, "puncturing" deep into the stroma or tube-like structures that often contained red blood cells. The sub-cellular localization of E-cadherin in the above structures, however, was primarily cytoplasmic. The mRNA level of E-cadherin in cell clusters overlying FMCLD was significantly higher than that in adjacent cells within the same duct. These findings suggest that aberrant expression of E-cadherin may contribute to cell motility and invasion. |
| Databáze: | OpenAIRE |
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