Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent
| Autor: | Bogdanov, M., Pyrshev, K., Yesylevskyy, S., Ryabichko, S., Boiko, V., Ivanchenko, P., Kiyamova, R., Guan, Z., Ramseyer, C., Dowhan, W. |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Gram-negative bacteria
Phospholipid Biochemistry Microbiology 03 medical and health sciences chemistry.chemical_compound Gram-Negative Bacteria Cardiolipin Escherichia coli Inner membrane Cell Shape Phospholipids Research Articles 030304 developmental biology Phosphatidylethanolamine 0303 health sciences Multidisciplinary biology Bilayer 030302 biochemistry & molecular biology Cell Membrane SciAdv r-articles Periplasmic space biology.organism_classification chemistry Cytoplasm Biophysics lipids (amino acids peptides and proteins) Research Article |
| Zdroj: | Science Advances |
| Popis: | The cell shape of Gram-negative bacteria is maintained metabolically by asymmetric lipid distribution in biogenic plasma membrane. The distribution of phospholipids across the inner membrane (IM) of Gram-negative bacteria is unknown. We demonstrate that the IMs of Escherichia coli and Yersinia pseudotuberculosis are asymmetric, with a 75%/25% (cytoplasmic/periplasmic leaflet) distribution of phosphatidylethanolamine (PE) in rod-shaped cells and an opposite distribution in E. coli filamentous cells. In initially filamentous PE-lacking E. coli cells, nascent PE appears first in the periplasmic leaflet. As the total PE content increases from nearly zero to 75%, cells progressively adopt a rod shape and PE appears in the cytoplasmic leaflet of the IM. The redistribution of PE influences the distribution of the other lipids between the leaflets. This correlates with the tendency of PE and cardiolipin to regulate antagonistically lipid order of the bilayer. The results suggest that PE asymmetry is metabolically controlled to balance temporally the net rates of synthesis and translocation, satisfy envelope growth capacity, and adjust bilayer chemical and physical properties. |
| Databáze: | OpenAIRE |
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