Susceptibility of Human Placenta Derived Mesenchymal Stromal/Stem Cells to Human Herpesviruses Infection

Autor: Cosetta Marchionni, Laura Bonsi, Antonella Rotola, Francesco Alviano, Valerio Leoni, Liliana Solimando, Giacomo Lanzoni, Dario Di Luca, Alessandro Ripalti, Gualtiero Alvisi, Simone Avanzi
Přispěvatelé: Avanzi S, Leoni V, Rotola A, Alviano F, Solimando L, Lanzoni G, Bonsi L, Di Luca D, Marchionni C, Alvisi G, Ripalti A
Rok vydání: 2013
Předmět:
Human cytomegalovirus
Viral Diseases
Placenta
viruses
Genetic enhancement
lcsh:Medicine
medicine.disease_cause
Pregnancy
Human herpesviruse
Cricetinae
Chlorocebus aethiops
Molecular Cell Biology
Bone Marrow and Stem Cell Transplantation
lcsh:Science
Cells
Cultured
Multidisciplinary
Stem Cells
Obstetrics and Gynecology
in vitro
Herpesviridae Infections
Hematology
gene therapy
Mesenchymal Stem Cell
Infectious Diseases
Medicine
Female
Disease Susceptibility
human term plscenta
Cellular Types
Stem cell
Epstein-Barr Virus
Research Article
Adult
bone marrow
Urology
Sexually Transmitted Diseases
Biology
Virus
Herpesviridae
NO
Viral vector
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Vero Cells
Transplantation
Genitourinary Infections
lcsh:R
Mesenchymal stem cell
Varicella zoster virus
Mesenchymal Stem Cells
Herpes Simplex
multiplex PCR
Embryo
Mammalian
medicine.disease
Virology
human cytoegalovirus amniotic membrane
Epstein-Barr Virus
processivity factor PPUL44
human term plscenta
stem cells
bone marrow
human cytoegalovirus amniotic membrane
multiplex PCR
gene therapy
in vitro
lcsh:Q
processivity factor PPUL44
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 8, p e71412 (2013)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0071412
Popis: Fetal membranes (FM) derived mesenchymal stromal/stem cells (MSCs) are higher in number, expansion and differentiation abilities compared with those obtained from adult tissues, including bone marrow. Upon systemic administration, ex vivo expanded FM-MSCs preferentially home to damaged tissues promoting regenerative processes through their unique biological properties. These characteristics together with their immune-privileged nature and immune suppressive activity, a low infection rate and young age of placenta compared to other sources of SCs make FM-MSCs an attractive target for cell-based therapy and a valuable tool in regenerative medicine, currently being evaluated in clinical trials. In the present study we investigated the permissivity of FM-MSCs to all members of the human Herpesviridae family, an issue which is relevant to their purification, propagation, conservation and therapeutic use, as well as to their potential role in the vertical transmission of viral agents to the fetus and to their potential viral vector-mediated genetic modification. We present here evidence that FM-MSCs are fully permissive to infection with Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), Varicella zoster virus (VZV), and Human Cytomegalovirus (HCMV), but not with Epstein-Barr virus (EBV), Human Herpesvirus-6, 7 and 8 (HHV-6, 7, 8) although these viruses are capable of entering FM-MSCs and transient, limited viral gene expression occurs. Our findings therefore strongly suggest that FM-MSCs should be screened for the presence of herpesviruses before xenotransplantation. In addition, they suggest that herpesviruses may be indicated as viral vectors for gene expression in MSCs both in gene therapy applications and in the selective induction of differentiation.
Databáze: OpenAIRE
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