Inhibitory and apoptosis-inducing effects of Newcastle disease virus strain AF2240 on mammary carcinoma cell line
Autor: | Fauziah Othman, Ismaila Ahmed, Yong Yoke Keong, Nizar Abd Manan, Umar Ahmad |
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Rok vydání: | 2014 |
Předmět: |
Article Subject
Newcastle disease virus lcsh:Medicine Apoptosis Breast Neoplasms Biology General Biochemistry Genetics and Molecular Biology Cell Movement Cell Line Tumor Cytotoxic T cell Humans MTT assay Cell Proliferation TUNEL assay General Immunology and Microbiology Cell growth lcsh:R General Medicine Cell cycle Virology Oncolytic virus Cancer research DNA fragmentation Female DNA Damage Research Article |
Zdroj: | BioMed Research International BioMed Research International, Vol 2015 (2015) |
ISSN: | 2314-6141 |
Popis: | Breast cancer is the malignant tumour that developed from cells of the breast and is the first leading cause of cancer death among women worldwide. Surgery, radiotherapy, and chemotherapy are the available treatments for breast cancer, but these were reported to have side effects. Newcastle disease virus (NDV) known asAvian paramyxovirustype-1 (APMV1) belongs to the genusAvulavirusin a family Paramyxoviridae. NDV is shown to be a promising anticancer agent, killing tumour cells while sparing normal cells unharmed. In this study, the oncolytic and cytotoxic activities of NDV AF2240 strain were evaluated on MDA-MB-231, human mammary carcinoma cell line, using MTT assay, and its inhibitory effects were further studied using proliferation and migration assays. Morphological and apoptotic-inducing effects of NDV on MD-MB-231 cells were observed using phase contrast and fluorescence microscopes. Detection of DNA fragmentation was done following terminal deoxyribonucleotide transferase-mediated Br-dUTP nick end labeling staining (TUNEL) assay, which confirmed that the mode of death was through apoptosis and was quantified by flow cytometry. Furthermore, analysis of cellular DNA content demonstrated that the virus caused an increase in the sub-G1 phase (apoptotic peak) of the cell cycle. It appears that NDV AF2240 strain is a potent anticancer agent that induced apoptosis in time-dependent manner. |
Databáze: | OpenAIRE |
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