Rapid and Sensitive PCR Detection ofVibrio trachuriPathogenic to Japanese Horse Mackerel (Trachurus japonicus)
| Autor: | Yoko Watanabe, Yusaku Suzuki, Tadayori Shimizu, Yoshihisa Iwamoto, Akinobu Kurita, Yasutake Yanagihara, Hirohisa Ohgami |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Immunology Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention chemistry.chemical_compound law Vibrionaceae Virology Animals Deoxyribonucleases Type II Site-Specific Polymerase chain reaction DNA Primers Vibrio Base Sequence biology Fishes biology.organism_classification Horse mackerel Molecular biology chemistry Trachurus japonicus Primer (molecular biology) DNA Bacteria |
| Zdroj: | Microbiology and Immunology. 39:1003-1006 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.1995.tb03290.x |
| Popis: | A polymerase chain reaction (PCR) method was performed for rapid and sensitive detection of pathogenic Vibrio trachuri isolated from cultured Japanese horse mackerel. A set of primers was selected from the base sequence of the Pst I fragment of T9210 chromosomal DNA and used for PCR detection of T9210. This PCR specifically amplified the DNAs from V. trachuri T9210, T9213, and T9216 but not of those other bacterial strains. PCR using a Pst I-1 primer set made it possible to detect 100 fg of T9210 DNA. The PCR method reported here may be useful for detection and identification of V. trachuri pathogenic to Japanese horse mackerel. |
| Databáze: | OpenAIRE |
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