Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM
| Autor: | Thomas E. Nielsen, Michael Rosholm Mortensen, Anne Louise Bank Kodal, Emiliano Cló, Jørgen Kjems, Per Franklin Nielsen, Kurt V. Gothelf, Thorbjørn B. Nielsen, Rasmus P. Thomsen |
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| Rok vydání: | 2019 |
| Předmět: |
Nanostructure
nanostructure bioconjugation Peptide 010402 general chemistry 01 natural sciences Catalysis chemistry.chemical_compound Microscopy Electron Transmission Labelling Cell Line Tumor antibodies Humans chemistry.chemical_classification Bioconjugation 010405 organic chemistry Oligonucleotide cyclic peptides General Chemistry DNA Cyclic peptide 0104 chemical sciences chemistry Immunoglobulin M Biophysics Peptides Rituximab Conjugate Protein Binding |
| Zdroj: | Nielsen, T B, Thomsen, R P, Mortensen, M R, Kjems, J, Nielsen, P F, Nielsen, T E, Kodal, A L B, Cló, E & Gothelf, K V 2019, ' Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM ', Angewandte Chemie International Edition, vol. 58, no. 27, pp. 9068-9072 . https://doi.org/10.1002/anie.201903134 |
| ISSN: | 1521-3773 |
| Popis: | The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis. |
| Databáze: | OpenAIRE |
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