Host cell egress of Brucella abortus requires BNIP3L ‐mediated mitophagy

Autor: Jérémy Verbeke, Youri Fayt, Lisa Martin, Oya Yilmaz, Jaroslaw Sedzicki, Angéline Reboul, Michel Jadot, Patricia Renard, Christoph Dehio, Henri‐François Renard, Jean‐Jacques Letesson, Xavier De Bolle, Thierry Arnould
Rok vydání: 2023
Předmět:
Zdroj: The EMBO Journal.
ISSN: 1460-2075
0261-4189
DOI: 10.15252/embj.2022112817
Popis: The facultative intracellular pathogen Brucella abortus interacts with several organelles of the host cell to reach its replicative niche inside the endoplasmic reticulum. However, little is known about the interplay between the intracellular bacteria and the host cell mitochondria. Here, we showed that B. abortus triggers substantive mitochondrial network fragmentation, accompanied by mitophagy and the formation of mitochondrial Brucella-containing vacuoles during the late steps of cellular infection. Brucella-induced expression of the mitophagy receptor BNIP3L is essential for these events and relies on the iron-dependent stabilisation of the hypoxia-inducible factor 1α. Functionally, BNIP3L-mediated mitophagy appears to be advantageous for bacterial exit from the host cell as BNIP3L depletion drastically reduces the number of reinfection events. Altogether, these findings highlight the intricate link between Brucella trafficking and the mitochondria during host cell infection.
Databáze: OpenAIRE
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