cDNA and deduced amino acid sequences of a peritrophic membrane glycoprotein, 'Peritrophin-48', from the larvae of Lucilia cuprina
| Autor: | Ross L. Tellam, Tony Vuocolo, Roger Pearson, Sandra Schorderet, George Riding, C.H. Eisemann |
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| Rok vydání: | 1998 |
| Předmět: |
Signal peptide
DNA Complementary Molecular Sequence Data Polymerase Chain Reaction Biochemistry Complementary DNA Animals Amino Acid Sequence Peritrophic matrix Molecular Biology Peptide sequence chemistry.chemical_classification Membrane Glycoproteins Base Sequence biology Diptera fungi Gene Expression Regulation Developmental biology.organism_classification Amino acid Membrane glycoproteins chemistry Membrane protein Lucilia cuprina Larva Insect Science biology.protein Insect Proteins Digestive System |
| Zdroj: | Insect Biochemistry and Molecular Biology. 28:99-111 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/s0965-1748(97)00103-3 |
| Popis: | The gut of most insects is lined with a semi-permeable peritrophic membrane (or peritrophic matrix) composed of chitin, proteoglycans and proteins. Despite the probable importance of the peritrophic membrane in facilitating the digestive process and protecting insects from invasion by micro-organisms and parasites, there has been little characterization of the specific components and their interactions within this acellular structure. Here we report the characterization of an integral peritrophic membrane glycoprotein, peritrophin-48, from the larvae of the fly Lucilia cuprina, a primary agent of cutaneous myiasis in sheep. Peritrophin-48 was purified from peritrophic membrane obtained by larval culture and its location within the peritrophic membrane determined by immuno-fluorescence and immuno-gold localizations. The cDNA coding for peritrophin-48 was cloned and sequenced. The deduced amino acid sequence codes for a protein of 375 amino acids containing an amino-terminal signal sequence followed by five similar, but non-identical domains, each approximately 65–70 amino acids in length and characterised by a specific register of six cysteines. The deduced amino acid sequence shows significant similarity to two other peritrophic membrane proteins, peritrophin-95 and peritrophin-44, from the same species. A reverse transcriptase-PCR approach indicated that there are several highly related peritrophin-48 genes expressed in each individual. Reverse transcriptase-PCR also demonstrated the expression of peritrophin-48 in all three larval instars and adults but not pupae or eggs. Peritrophin-48 was expressed only by the cardia and by the larval midgut. A simple structural model of a basic unit of a type 2 peritrophic membrane is presented. |
| Databáze: | OpenAIRE |
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