Trypanosoma brucei brucei: the catabolism of glycolytic intermediates by digitonin-permeabilized bloodstream trypomastigotes and some aspects of regulation of anaerobic glycolysis
| Autor: | Job K. Kiaira, R. M. Njogu |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Glycerol
Cell Membrane Permeability Trypanosoma brucei brucei Digitonin Trypanosoma brucei Biochemistry chemistry.chemical_compound Adenosine Triphosphate Salicylamides Animals Glycolysis Anaerobiosis Pyruvates biology Catabolism Substrate (chemistry) biology.organism_classification Salicylhydroxamic acid Rats Adenosine Diphosphate Glucose Trypanosomiasis African chemistry Anaerobic glycolysis Glycerophosphates |
| Zdroj: | The International journal of biochemistry. 20(10) |
| ISSN: | 0020-711X |
| Popis: | 1. 1. The production of pyruvate, glycerol and glycerol-3-phosphate by intact and digitonin-permeabilized Trypanosoma brucei brucei has been studied with glucose or the glycolytic intermediates as substrates. 2. 2. Under aerobic conditions hexosephosphates gave maximal glycolysis in the presence of 40–60μg digitonin/10 8 trypanosomes while the triosephosphates gave it at 20–30μg digitonin/10 8 trypanosomes. 3. 3. In the presence of salicylhydroxamic acid, and the glycolytic intermediates, permeabilized trypanosomes produced equimolar amounts of pyruvate and glycerol-3-phosphate and no glycerol. Under the same conditions, glucose catabolism produced glycerol in addition to pyruvated and glycerol-3-phosphate. 4. 4. In the presence of salicylhydroxamic acid and ATP or ADP intact trypanosomes produced equimolar amounts of pyruvate and (glycerol plus glycerol-3-phosphate) with glucose as substrate. 5. 5. A carrier for ATP and ADP at the glycosomal membrane is implicated. 6. 6. It is apparent that glycerol formation is regulated by the ATP/ADP ratio and that it needs intact glycosomal membrane and the presence of glucose. |
| Databáze: | OpenAIRE |
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