NFAM1, an immunoreceptor tyrosine-based activation motif-bearing molecule that regulates B cell development and signaling
| Autor: | Tadahiro Suenaga, Toshio Kitamura, Makoto Ohtsuka, Sho Yamasaki, Tadashi Yokosuka, Takashi Saito, Noriko Arase, Ritsuko Shiina, Hisashi Arase, Arata Takeuchi, Daiju Sakurai, Makio Iwashima, Hideshige Moriya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary medicine.medical_treatment Amino Acid Motifs Molecular Sequence Data Syk Bone Marrow Cells Mice Transgenic Receptors Cell Surface Biology Cell Line Mice Membrane Microdomains Immunoreceptor tyrosine-based activation motif medicine Animals Humans Amino Acid Sequence Tyrosine Cloning Molecular B cell Bone Marrow Transplantation B-Lymphocytes Multidisciplinary Chimera Receptor Aggregation Membrane Proteins NFAT Cell Differentiation Biological Sciences Molecular biology Cell biology Mice Inbred C57BL Cytokine medicine.anatomical_structure Cell culture Phosphorylation Signal Transduction |
| Zdroj: | ResearcherID |
| Popis: | A functional cDNA cloning system was developed by using a retrovirus library encoding CD8-chimeric proteins and a nuclear factor of activated T cells (NFAT)-GFP reporter cell line to identify molecules inducing NFAT activation. By using this strategy, NFAT activating molecule 1 (NFAM1) was cloned as an immunoreceptor tyrosine-based activation motif (ITAM)-bearing cell surface molecule belonging to the Ig superfamily and is predominantly expressed in spleen B and T cells. NFAM1 crosslinking induced ITAM phosphorylation, ZAP-70/Syk recruitment, NFAT activation, and cytokine production.In vivooverexpression of NFAM1 in bone marrow chimeras and transgenic mice induced severe impairment of early B cell development in an ITAM-dependent manner. In NFAM1-expressing B cells, B cell antigen receptor stimulation induced NFAM1 translocation to lipid raft, and NFAM1 co-crosslinking augmented B cell antigen receptor signaling. The results suggest that NFAM1 modulates B cell signaling through its ITAM, which regulates B cell development. |
| Databáze: | OpenAIRE |
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