Topical CpG Oligodeoxynucleotide Adjuvant Enhances the Adaptive Immune Response against Influenza A Infections
Autor: | Wing Ki Cheng, Adam William Plumb, Jacqueline Cheuk-Yan Lai, Ninan Abraham, Jan Peter Dutz |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine CpG Oligodeoxynucleotide medicine.medical_treatment Immunology Priming (immunology) T cell memory Biology medicine.disease_cause Epitope 03 medical and health sciences 0302 clinical medicine influenza vaccines medicine Influenza A virus Immunology and Allergy Cytotoxic T cell Original Research antibody production Acquired immune system Virology skin vaccination 3. Good health CpG oligodeoxynucleotide 030104 developmental biology medicine.anatomical_structure lcsh:RC581-607 Adjuvant Memory T cell 030215 immunology |
Zdroj: | Frontiers in Immunology Frontiers in Immunology, Vol 7 (2016) |
ISSN: | 1664-3224 |
Popis: | Current influenza vaccines generate humoral immunity, targeting highly variable epitopes and thus fail to achieve long-term protection. T cells recognize and respond to several highly conserved epitopes across influenza serotypes. A strategy of raising strong cytotoxic T cell memory responses to epitopes conserved across serotypes would provide cross serotype protection, eliminating the need for annual vaccination. We explored the adjuvant potential of epicutaneous (ec) and subcutaneous (sc) delivery of CpG oligodeoxynucleotide in conjunction with sc protein immunization to improve protection against influenza A virus (IAV) infections using a mouse model. We found enhanced long-term protection with epicutaneous CpG ODN (ecCpG) compared to subcutaneous CpG ODN (scCpG) as demonstrated by reduced viral titers in the lungs. This correlated with increased antigen-specific CD8 T cells in the airways and the lungs. The memory T cell response after immunization with ecCpG adjuvant was comparable to memory response by priming with IAV infection in the lungs. In addition, ecCpG was more efficient than scCpG in inducing the generation of IFN-γ producing CD4 T cells. The adjuvant effect of ecCpG was accompanied with its ability to modulate tissue-homing molecules on T cells that may direct them to the site of infection. Together, this work provides evidence for using ecCpG to induce strong antibody and memory T cell responses to confer protection against IAV infection. |
Databáze: | OpenAIRE |
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