Chimeric swine vesicular disease viruses produced by fusion PCR: a new method for epitope mapping
| Autor: | C. H. Leendertse, F. van Poelwijk, Aldo Dekker, R.J.M. Moormann, J. M. J. Rebel |
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| Rok vydání: | 2000 |
| Předmět: |
Picornavirus
medicine.drug_class Swine Epitope mapping Molecular Sequence Data Monoclonal antibody medicine.disease_cause Antibodies Viral Polymerase Chain Reaction Epitope law.invention Swine Vesicular Disease law Antibody Specificity Neutralization Tests Virology medicine Animals Instituut voor Dierhouderij en Diergezondheid Antigens Viral Swine vesicular disease Polymerase chain reaction Enterovirus biology ID-Lelystad Base Sequence Antibodies Monoclonal Sequence Analysis DNA biology.organism_classification Molecular biology Chimeric virus ID Lelystad Swine Vesicular Disease Virus ID-Lelystad Instituut voor Dierhouderij en Diergezondheid ID Lelystad Institute for Animal Science and Health Mutation WIAS Monoclonal antibodies Institute for Animal Science and Health Epitope Mapping |
| Zdroj: | Journal of Virological Methods, 86, 131-141 Journal of Virological Methods 86 (2000) |
| ISSN: | 0166-0934 |
| Popis: | A new method of epitope mapping based on chimeric swine vesicular disease (SVD) viruses produced by fusion PCR (polymerase chain reaction). Seven out of 16 neutralising and non-neutralising newly produced monoclonal antibodies (MAbs) discriminated between SVD isolate ITL/1/66 and NET/1/92. Using fusion PCR eight chimeric viruses were produced containing different supplementary pieces of the P1 region of both parent strains. Using these chimeric viruses we were able to map the epitope regions recognised by these seven neutralising and non-neutralising Mabs. This new method, using chimeric viruses produced by fusion PCR, is particularly valuable for the epitope mapping of non-neutralising MAbs. |
| Databáze: | OpenAIRE |
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