Kinetics of chicken embryonic thymocyte developmentin ovo and in organ culture
| Autor: | Richard L. Boyd, Natalie J. Davidson, Chen-lo H. Chen |
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| Rok vydání: | 1992 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD3 Complex CD8 Antigens CD3 T cell Immunology Population Receptors Antigen T-Cell Gene Expression Chick Embryo Thymus Gland In ovo Organ culture Immunophenotyping Organ Culture Techniques medicine Animals Immunology and Allergy education education.field_of_study biology Cell Differentiation Receptors Antigen T-Cell gamma-delta T lymphocyte Flow Cytometry Cell biology Thymocyte medicine.anatomical_structure CD4 Antigens biology.protein CD8 |
| Zdroj: | European Journal of Immunology. 22:1429-1435 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830220615 |
| Popis: | Thymocyte development was monitored in an embryonic thymus organ culture system to establish a model in the chicken in which the functional nature of the thymic microenvironment could be assessed. Thymus lobes were removed from 10-day-old embryos and cultured for 2-10 days. Cell yield increased to a maximum in 4-8 days of culture with a corresponding decrease in average cell size. An initial thymocyte population of predominantly CD3-CD4-CD8- cells gave rise to all CD3/CD4/CD8-defined subpopulations in vitro, maintaining high levels of CD3-CD4-CD8+ and CD3+CD4-CD8+ cells and a low representation of CD3-CD4+CD8-, CD3+CD4+CD8-, CD3-CD4+CD8+ and CD3+CD4+CD8+ thymocytes. This is the first observation of a CD3-CD4+CD8- population in the chicken. Developmental kinetics of CD3+ cells were similar to that in the embryo, suggesting that the in vitro environment is sufficient to promote and maintain thymocyte maturation. Thymocytes of both the gamma delta and alpha beta T cell receptor (TcR) lineages developed in that order, confirming in ovo data and the lineage potential of the first wave of thymocyte precursors. One unusual finding was a relative accumulation of gamma delta TcR+ thymocytes in culture, incorporating all CD4/CD8 subsets, including a previously undetected population, CD4+CD8-. This may indicate a favorable developmental environment or simply a lack of normal cellular emigration. A detailed comparison with T cell development in the embryo demonstrated that the chicken thymus organ culture system reflects thymic events in ovo during a limited time period and thus should prove useful in the identification of functionally relevant thymic molecules. |
| Databáze: | OpenAIRE |
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