The pseudogene-derived long noncoding RNA SFTA1P is down-regulated and suppresses cell migration and invasion in lung adenocarcinoma
| Autor: | Chenchen Wei, Fengqi Nie, Yaqiong Xiong, Xuefei Shi, Rui Xia, Hua Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lung Neoplasms RNA Untranslated Pseudogene Down-Regulation Adenocarcinoma of Lung Biology Adenocarcinoma Genome 03 medical and health sciences Mice Cell Movement Cell Line Tumor medicine Animals Humans Gene RC254-282 Mice Inbred BALB C Microarray analysis techniques Neoplasms. Tumors. Oncology. Including cancer and carcinogens RNA Cell migration General Medicine medicine.disease Long non-coding RNA 030104 developmental biology A549 Cells Cancer research Heterografts Female RNA Long Noncoding Pseudogenes |
| Zdroj: | Tumor Biology, Vol 39 (2017) |
| ISSN: | 1423-0380 |
| Popis: | Pseudogenes were once considered to be genomic fossils without biological function. Interestingly, recent evidence showed that a lot of pseudogenes are transcribed in human cancers, and their alterations contribute to multiple cancer development and progression. It is apparent that many pseudogenes transcribe noncoding RNAs and contribute to the role noncoding genome plays in human cancers. On this basis, some pseudogene transcripts are currently ranked among the classes of long noncoding RNAs. In this study, we identified a new pseudogene-derived long noncoding RNA termed SFTA1P by analyzing the microarray data of non–small cell lung cancer from Gene Expression Omnibus datasets. We found that SFTA1P expression was significantly decreased in non–small cell lung cancer tissues compared with normal tissues in non–small cell lung cancer microarray data. Moreover, decreased SFTA1P expression is only correlated with lung adenocarcinoma patients’ poor survival time but not with lung squamous cell carcinoma patients’ survival. In addition, gain-of-function studies including growth curves, migration, invasion assays, and in vivo studies were performed to verify the tumor suppressor role of SFTA1P in non–small cell lung cancer. Finally, the potential underlying pathways involved in SFTA1P were investigated by analyzing the SFTA1P-correlated genes in The Cancer Genome Atlas lung adenocarcinoma and normal tissues RNA sequencing data. Taken together, these findings demonstrate that pseudogene-derived long noncoding RNA SFTA1P exerts the tumor suppressor functions in human lung adenocarcinoma. Our investigation reveals the novel roles of pseudogene in lung adenocarcinoma, which may serve as a new target for lung adenocarcinoma diagnosis and therapy. |
| Databáze: | OpenAIRE |
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