Changes in bacterial community and abundance of functional genes in paddy soil with cry1Ab transgenic rice
| Autor: | Ming-ji Wu, Feng Wang, Ya-na Song, Zai-jie Chen, Gang Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Field experiment
Agriculture (General) microbial functional gene Functional genes Plant Science Biology Biochemistry bacterial community S1-972 Food Animals Abundance (ecology) Illumina MiSeq Platform Cultivar Ecology fungi food and beverages 16S ribosomal RNA Genetically modified rice UniFrac Agronomy cry1Ab transgenic rice Animal Science and Zoology Alpha diversity real-time PCR Agronomy and Crop Science Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 20, Iss 6, Pp 1674-1686 (2021) |
| ISSN: | 2095-3119 |
| Popis: | A field experiment involving cry1Ab transgenic rice (GM) and its parental non-cry1Ab rice (M) has been on-going since 2014. The diversity of the bacterial communities and the abundance of the microbial functional genes which drive the conversion of nitrogen in paddy soil were analyzed during the growth period of rice in the fifth year of the experiment, using 16S rRNA-based Illumina MiSeq and real-time PCR on the amoA, nirS and nirK genes. The results showed no differences in the alpha diversity indexes of the bacterial communities, including Chao1, Shannon and Simpson, between the fields cultivated with line GM and cultivar M at any of the growth stages of rice. However, the bacterial communities in the paddy soil with line GM were separated from those of paddy soil with cultivar M at each of the growth stages of rice, based on the unweighted UniFrac NMDS or PCoA. In addition, the analyses of ADONIS and ANOSIM, based on the unweighted UniFrac distance, indicated that the above separations between line GM and cultivar M were statistically significant (P |
| Databáze: | OpenAIRE |
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