| Autor: |
Lujie Ma, Le Wang, Mengdi Gao, Xinjie Zhang, Xiangdong Zhao, Debao Xie, Jing Zhang, Zhen Wang, Lifeng Hou, Fanli Zeng |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 36(11) |
| ISSN: |
1530-6860 |
| Popis: |
RNA polymerase II (RNAPII) is an essential machinery for catalyzing mRNA synthesis and controlling cell fate in eukaryotes. Although the structure and function of RNAPII have been relatively defined, the molecular mechanism of its assembly process is not clear. The identification and functional analysis of assembly factors will provide new understanding to transcription regulation. In this study, we identify that RTR1, a known transcription regulator, is a new multicopy genetic suppressor of mutants of assembly factors Gpn3, Gpn2, and Rba50. We demonstrate that Rtr1 is directly required to assemble the two largest subunits of RNAPII by coordinating with Gpn3 and Npa3. Deletion of RTR1 leads to cytoplasmic clumping of RNAPII subunit and multiple copies of RTR1 can inhibit the formation of cytoplasmic clump of RNAPII subunit in gpn3-9 mutant, indicating a new layer function of Rtr1 in checking proper assembly of RNAPII. In addition, we find that disrupted activity of Rtr1 phosphatase does not trigger the formation of cytoplasmic clump of RNAPII subunit in a catalytically inactive mutant of RTR1. Based on these results, we conclude that Rtr1 cooperates with Gpn3 and Npa3 to assemble RNAPII core. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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