Bioinformatics analysis of ubiquitin expression protein gene from Heterodera latipons
Autor: | Ahmed A.M. Dawabah, Hamzeh A. Lafi, Saleh AlNadhari, Monther T. Sadder, Luma Al-Banna |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Sequence analysis 01 natural sciences Article Mediterranean cereal cyst nematode Homology (biology) 03 medical and health sciences Ubiquitin Heterodera latipons Gene family Gene lcsh:QH301-705.5 chemistry.chemical_classification biology Protein DNA biology.organism_classification Amino acid Open reading frame 030104 developmental biology chemistry Biochemistry lcsh:Biology (General) biology.protein General Agricultural and Biological Sciences Nucleotide 010606 plant biology & botany |
Zdroj: | Saudi Journal of Biological Sciences, Vol 26, Iss 7, Pp 1463-1467 (2019) Saudi Journal of Biological Sciences |
Popis: | Ubiquitin expression protein DNA clone (Hl-UBI) was isolated from Heterodera latipons collected from North Jordan. Its sequence of 285 nucleotides was also determined and deposited in the GeneBank. The 285-bp open reading frame coded for 76-amino acid protein having two domains; the ubiquitin domain and the C terminal extension. The first 59 amino acids were predicted with the ubiquitin domain with identity percentages of 78% to ubiquitin of H. schachtii, 77% to polyubiquitin of Globodera pallida, 74% to ubiquitin of Globodera rostochiensis and 72% to ubiquitin of Heterodera glycines. The other domain at the C-terminus, containing 17 amino acids, showed no homology to any known protein. Sequence analysis showed a calculated encoding amino acids molecular weight of 8.77 kDa, theoretical isoelectric point = 4.76, negatively charged residues = 12, positively charged residues = 9, extinction coefficient = 1490, estimated half-life = 30 h, instability index = 32.51 and grand average of hydropathicity = −0.537. The demonstrated subcellular localization analysis of cytoplasm, cell nucleus, mitochondrion, cell skeleton and plasma membrane of Hl-UBI protein occupied about 52.20, 21.70, 17.40, 4.30 and 4.30%, respectively. Sequence, homology and structural analysis confirmed that Hl-UBI gene was highly conserved during evolution and belonged to ubiquitin gene family. Keywords: DNA, Mediterranean cereal cyst nematode, Nucleotide, Protein |
Databáze: | OpenAIRE |
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