Quantum-dot-embedded silica nanotubes as nanoprobes for simple and sensitive DNA detection
| Autor: | Chia Chun Chen, Jinn Shiun Chen, Yi Hsin Liu, Yi-Chun Wu, Chien Ying Chen, Hsiao Ju Chien, Yu Feng Huang, Yi Yun Tsai |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Complementary Microscope Materials science Silicon dioxide Nanoprobe Bioengineering Nanotechnology law.invention chemistry.chemical_compound Nucleic acid thermodynamics Microscopy Electron Transmission law Confocal microscopy Quantum Dots Microscopy General Materials Science Particle Size Electrical and Electronic Engineering Fluorescent Dyes Nanotubes Rhodamines Mechanical Engineering Nucleic Acid Hybridization DNA General Chemistry Carbocyanines Silicon Dioxide Fluorescence Spectrometry Fluorescence Microscopy Fluorescence chemistry Mechanics of Materials Quantum dot Adsorption DNA Probes Fluorescein-5-isothiocyanate |
| Zdroj: | Nanotechnology. 22:155102 |
| ISSN: | 1361-6528 0957-4484 |
| DOI: | 10.1088/0957-4484/22/15/155102 |
| Popis: | We have developed a new technique using fluorescent silica nanotubes for simple and sensitive DNA detection. The quantum-dot-embedded silica nanotubes (QD-SNTs) were fabricated by a sol-gel reaction using anodic aluminum silica oxide (AAO) as a template. The fluorescent QD-SNTs of different colors were then immobilized with single-stranded DNA and used as nanoprobes for DNA detection. The optical and structural properties of QD-SNT nanoprobes were examined using photoluminescence spectroscopy, confocal microscopy and transmission electron microscopy (TEM). The QD-SNT nanoprobes were applied to detect dye-labeled target DNA in a solution phase. The obvious color change of the QD-SNT nanoprobes was observed visually under a simple microscope after the successful detection with target DNA. The quantitative analyses indicated that ∼ 100 attomole of target DNA in one nanoprobe can generate a distinguishable and observable color change. The detection results also demonstrated that our assay exhibited high specificity, high selectivity and very low nonspecific adsorption. Our simple DNA assay based on QD-SNT nanoprobes is expected to be quite useful for the needs of fast DNA screening and detection applications. |
| Databáze: | OpenAIRE |
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