Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions
| Autor: | Susmita Bagchi, Cristina Montagna, Michal Bejerano-Sagie, Nicole E. Patterson, Gary L. Goldberg, Aaron Golden, Jenna Z. Marcus, John S. Condeelis, Diana Connolly, Vladislav V. Verkhusha, Ved P. Sharma |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Carcinogenesis Septin 9 Breast Neoplasms Biology Matrix metalloproteinase migration Septin medicine.disease_cause Article metalloproteinases Extracellular matrix Focal adhesion 03 medical and health sciences breast cancer 0302 clinical medicine Downregulation and upregulation oncogene Cell Movement Tumor Microenvironment Genetics medicine Humans Protein Isoforms Neoplasm Invasiveness Secretion Mammary Glands Human Molecular Biology Focal Adhesions Tumor microenvironment invasion Matrix Metalloproteinases Extracellular Matrix Up-Regulation Cell biology 030104 developmental biology 030220 oncology & carcinogenesis MCF-7 Cells MMP3 Septins |
| Zdroj: | Oncogene |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | The cytoskeletal interacting protein Septin 9 (SEPT9), a member of the septin gene family, has been proposed to have oncogenic functions. It is a known hot spot of retroviral tagging insertion and a fusion partner of both de novo and therapy-induced mixed lineage leukemia (MLL). Of all septins, SEPT9 holds the strongest link to cancer, especially breast cancer. Murine models of breast cancer frequently exhibit SEPT9 amplification in the form of double minute chromosomes, and about 20% of human breast cancer display genomic amplification and protein over expression at the SEPT9 locus. Yet, a clear mechanism by which SEPT9 elicits tumor-promoting functions is lacking. To obtain unbiased insights on molecular signatures of SEPT9 upregulation in breast tumors, we overexpressed several of its isoforms in breast cancer cell lines. Global transcriptomic profiling supports a role of SEPT9 in invasion. Functional studies reveal that SEPT9 upregulation is sufficient to increase degradation of the extracellular matrix, while SEPT9 downregulation inhibits this process. The degradation pattern is peripheral and associated with focal adhesions (FAs), where it is coupled with increased expression of matrix metalloproteinases (MMPs). SEPT9 overexpression induces MMP upregulation in human tumors and in culture models and promotes MMP3 secretion to the media at FAs. Downregulation of SEPT9 or chemical inhibition of septin filament assembly impairs recruitment of MMP3 to FAs. Our results indicate that SEPT9 promotes upregulation and both trafficking and secretion of MMPs near FAs, thus enhancing migration and invasion of breast cancer cells. |
| Databáze: | OpenAIRE |
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