Nitric oxide modulates metalloproteinase-2, collagen deposition and adhesion rate after polypropylene mesh implantation in the intra-abdominal wall

Autor: Wagner Marcondes, Irineu Tadeu Velasco, Ana Iochabel Soares Moretti, Francisco José Pellegrini de Souza Pinto, Marcia C. Jurado, Vivian Cury, Heraldo Possolo de Souza
Rok vydání: 2012
Předmět:
Male
medicine.medical_specialty
Materials science
Interleukin-1beta
Biomedical Engineering
Adhesion (medicine)
Nitric Oxide Synthase Type II
Biocompatible Materials
Tissue Adhesions
Matrix metalloproteinase
Nitric Oxide
Polypropylenes
Biochemistry
Nitric oxide
Biomaterials
Abdominal wall
chemistry.chemical_compound
Mice
Random Allocation
Peritoneum
Internal medicine
parasitic diseases
Materials Testing
medicine
Animals
Molecular Biology
Inflammation
Mice
Knockout
Wound Healing
biology
Abdominal Wall
Nitric oxide synthase 2
General Medicine
Prostheses and Implants
Surgical Mesh
medicine.disease
Surgery
Mice
Inbred C57BL
Endocrinology
Surgical mesh
medicine.anatomical_structure
chemistry
Matrix Metalloproteinase 9
METALOPROTEINASES
biology.protein
Matrix Metalloproteinase 2
Implant
Collagen
Biotechnology
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Popis: Prosthetic meshes are commonly used to correct abdominal wall defects. However, the inflammatory reaction induced by these devices in the peritoneum is not completely understood. We hypothesized that nitric oxide (NO), produced by nitric oxide synthase 2 (NOS2) may modulate the response induced by mesh implants in the abdominal wall and, consequently, affect the outcome of the surgical procedure. Polypropylene meshes were implanted in the peritoneal side of the abdominal wall in wild-type and NOS2-deficient (NOS2(-/-)) mice. After 15 days tissues around the mesh implant were collected, and inflammatory markers (the cytokine interleukin 1β (IL-1β) and NO) and tissue remodeling (collagen and metalloproteinases (MMP) 2 and 9) were analyzed. The lack of NOS2-derived NO induced a higher incidence of visceral adhesions at the mesh implantation site compared with wild-type mice that underwent the same procedure (P
Databáze: OpenAIRE
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