Detection and molecular characterization of tick-borne pathogens infecting sheep and goats in Blue Nile and West Kordofan states in Sudan
| Autor: | Ehab Mossaad, Huanping Guo, Xuenan Xuan, Abdalla Mohamed Ibrahim, Jixu Li, Keisuke Suganuma, Peter Musinguzi, Paul Franck Adjou Moumouni, Aaron Edmond Ringo, Artemis Efstratiou, Yang Gao, Seung-Hun Lee, Tamador Elkhansa Elnour Angara, Ahmed Ali Ismail, Noboru Inoue, Mingming Liu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Anaplasmosis Veterinary medicine Anaplasma 030231 tropical medicine Prevalence Sheep Diseases Ehrlichia ruminantium Polymerase Chain Reaction Microbiology Sudan 03 medical and health sciences Ticks 0302 clinical medicine Babesiosis Theileria parasitic diseases RNA Ribosomal 18S medicine Animals Ovis Tick-borne disease Goat Diseases Sheep Molecular epidemiology biology business.industry Goats Anaplasma ovis medicine.disease biology.organism_classification 16S ribosomal RNA Theileriasis Africa Western 030104 developmental biology Infectious Diseases Tick-Borne Diseases Insect Science Parasitology Livestock business |
| Zdroj: | Ticks and Tick-borne Diseases. 9:598-604 |
| ISSN: | 1877-959X |
| Popis: | Tick-borne pathogens (TBPs) are common in livestock of sub-Saharan Africa. However, information regarding TBPs in sheep and goats in Sudan is limited. In this study, 178 blood samples of sheep and goats in Blue Nile and West Kordofan states were investigated for TBPs using PCR. Overall, 110 (61.8%) samples were found to be infected with at least one of the following pathogens: Anaplasma ovis, Theileria ovis, and Ehrlichia ruminantium. Babesia ovis and T. lestoquardi were not identified. A. ovis was the most prevalent pathogen (n = 107, 60.1%), followed by T. ovis (n = 23, 12.9%) and E. ruminantium (n = 1, 0.6%). The prevalence rates of A. ovis and T. ovis were significantly higher in sheep than in goats. Phylogenetic analysis of T. ovis 18S rRNA and A. ovis msp4, groEL, and 16S rRNA, revealed that the pathogens identified in this study are clustered together, indicating similar molecular characteristics. Additionally, phylogenetic analysis of E. ruminantium pCS20 revealed that E. ruminantium in this study belong to the West Africa group, and different to E. ruminantium previously identified in ticks from Sudan. We concluded that TBPs are highly prevalent in the study area and continuous monitoring of TBPs in sheep and goats in Sudan is highly required. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect